Moore Notes 11 13 13

Group Call

• Participants: Katie, Tom, Josh, Ladan, Guillaume

• PICRUSTs vs. Shotmap analysis (both with Kegg Orthology groups (KOs))
  • Filtered: only using KOs present in PICRUSTs results http://edhar.genomecenter.ucdavis.edu/~gjospin/picrust_test/picrust_test/filtered_results/
    • Hamming distance between samples is good (presence-absence)
      • Check what families these are (e.g., via Patrick's MetaCyc analysis)
    • But abundance vectors are only weakly correlated (Spearman rho)
  • Top 100: most abundant KOs from PICRUSTs http://edhar.genomecenter.ucdavis.edu/~gjospin/picrust_test/picrust_test/top100/
    • Correlations are not better for these KOs
  • If shotmap is more sensitive, then using stricter thresholds for read classification should improve correlation with PICRUSTs (and Jack's metagenome annotation)
    • Tom and Guillaume will check this and report back next week

• Shotmap paper
  • Wrapping up analyses in next few weeks
  • Then finish writing
  • Patrick could contribute MetaCyc annotation results

• ISME 2014: South Korea
  • Any one going?

• Next call: Guillaume and Josh

• Two weeks from now: Ladan