Discussion of Huse et al. and Dethlefsen et al. 2008 tag pyrosequencing papers
0. We are going to put together a roadmap for metagenomic research (to connect what people are doing).
1. Why aren't there phylogenies in any of these papers? Could we use AMPHORA to build a tree?
2. Next time, let's read reference 40 and 44 in Dethlefsen et al. 2008. Also Hamady et al. 2008. Finally, Mardis et al. 2008.
3. How you can get 100s of samples in one 454 run. You can put a bar code on your sequence. Is this true for both tag sequencing and metagenomic (shotgun) sequencing?