Mike Barnkob:Protocols/Mediums

Media

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Recipes for media.

R0

• 500 ml of RPMI
• 5 ml of Pen/strep (100 U/mL penicillin and 100 ug/mL streptomycin)
• 2 mM L-glutamine

R10

• 500 ml of RPMI
• 50 ml of FCS
• 5 ml of Pen/strep (100 U/mL penicillin and 100 ug/mL streptomycin)
• 2 mM L-glutamine

R10 for T cells

• 500 ml of RPMI
• 50 ml of FCS
• 5 ml of Pen/strep (100 U/mL penicillin and 100 ug/mL streptomycin)
• 2 mM L-glutamine
• 50 μM B2-M (500μL of 50mM solution)

D10

• 500 ml of DMEM
• 50 ml of FCS
• 5 ml of Pen/strep (100 U/mL penicillin and 100 ug/mL streptomycin)
• 2 mM L-glutamine

FACS buffer

• 500 ml of DPBS
• 2mM EDTA
• 50 ml of FCS