Mike Barnkob:Protocols/Mediums

From OpenWetWare
Jump to: navigation, search

Media

Front page

Recipes for media.

R0

  • 500 ml of RPMI
  • 5 ml of Pen/strep (100 U/mL penicillin and 100 ug/mL streptomycin)
  • 2 mM L-glutamine

R10

  • 500 ml of RPMI
  • 50 ml of FCS
  • 5 ml of Pen/strep (100 U/mL penicillin and 100 ug/mL streptomycin)
  • 2 mM L-glutamine

R10 for T cells

  • 500 ml of RPMI
  • 50 ml of FCS
  • 5 ml of Pen/strep (100 U/mL penicillin and 100 ug/mL streptomycin)
  • 2 mM L-glutamine
  • 50 μM B2-M (500μL of 50mM solution)

D10

  • 500 ml of DMEM
  • 50 ml of FCS
  • 5 ml of Pen/strep (100 U/mL penicillin and 100 ug/mL streptomycin)
  • 2 mM L-glutamine

FACS buffer

  • 500 ml of DPBS
  • 2mM EDTA
  • 50 ml of FCS