Michael Eisen Lab:Reagents:PBS

Phosphate Buffered Saline

Usage

PBS is an isotonic buffer for cells.

Preparation

Purchase in Solution

This should be used as a last resort.

• PBS is available as 10X Solution from the Stanley Hall stock room

Prepare from tablets

• PBS is ideally prepared from PBS tablets or constituent salts (see wikipedia page).
  • Sterilize solution with sterile filter.

1X PBS

1x PBS is 137 mM NaCl, 12 mM Phosphate, 2.7 mM KCl, pH 7.4

10X PBS Stock Solution

1. Combine the following:
   • 80 g NaCl
   • 2 g KCl
   • 14.4 g Na₂HPO₄ (dibasic anhydrous) OR 18.1 g Na₂HPO₄ · 2H₂O (dibasic dihydrate) OR 27.2 g Na₂HPO₄ · 7H₂O (dibasic heptahydrate)
   • 2.4 g KH₂PO₄ (monobasic anhydrous)
   • 800 mL distilled H₂O
2. Adjust pH to 7.4 with HCl
3. Add H₂O to 1L
4. Autoclave for 20 minutes on liquid cycle or sterile filter. Store at RT.

Storage

• PBS tablets on chemical shelf
• PBS stock solutions on benchtops

Wikipedia.org PBS Page