Michael Eisen Lab:Reagents:PBS

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Phosphate Buffered Saline


PBS is an isotonic buffer for cells.


Purchase in Solution

This should be used as a last resort.

  • PBS is available as 10X Solution from the Stanley Hall stock room

Prepare from tablets

  • PBS is ideally prepared from PBS tablets [1] or constituent salts (see wikipedia page).
    • Sterilize solution with sterile filter.


1x PBS is 137 mM NaCl, 12 mM Phosphate, 2.7 mM KCl, pH 7.4

10X PBS Stock Solution

  1. Combine the following:
    • 80 g NaCl
    • 2 g KCl
    • 14.4 g Na2HPO4 (dibasic anhydrous) OR 18.1 g Na2HPO4 · 2H2O (dibasic dihydrate) OR 27.2 g Na2HPO4 · 7H2O (dibasic heptahydrate)
    • 2.4 g KH2PO4 (monobasic anhydrous)
    • 800 mL distilled H2O
  2. Adjust pH to 7.4 with HCl
  3. Add H2O to 1L
  4. Autoclave for 20 minutes on liquid cycle or sterile filter. Store at RT.


  • PBS tablets on chemical shelf
  • PBS stock solutions on benchtops

Wikipedia.org PBS Page

  1. Sigma P4417