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  • DMSO (dimethyl sulfoxide): liquid in cabinet under chemical hood
  • Kanamycin: powder in shelves above electrophoresis area
  • Hygromycin: 50mg/mL liquid in 4°C deli fridge near hoods
  • X-gal: 50mg/mL liquid in -20°C near hoods. Dilute in dimethylformadide (under chemical hood) to 40μg/mL to make working solution, which should be stored wrapped in aluminum foil (X-gal is very light sensitive) at -20°C. Use 50μL (~80ng/mL) per plate for blue/white screening of E. coli.