YPD (1x) (1L) Agar
• Begin with approx. 700 mls of DI H2O • Weigh out and add ingredients with constant stirring:
- Bacto Yeast Extract 10g
- Bacto Peptone 20g
• Weigh out and add with stirring:
- Bacto Agar 20g
• Bring up to 900 mLs with DI H2O • Separate YP into two 1L bottles of 450 mLs each • Weigh out 20g of Glucose/Dextrose and add to 100 mLs of DI H2O • Autoclave separately • Allow to cool in 50û water bath • Combine each 450 mL YP with 50 mLs glucose solution
Verified 6/1/2005 by MaryAnn Hartman; Methods in Yeast Genomics, Cold Spring Harbor, 2000 ed.