McClean:YPG

From OpenWetWare

Overview

YPG is a complex medium containing a nonfermentable carbon source (glycerol) that does not support the growth of petite mutants.

Reagents

  • Bacto-yeast extract
  • Bacto-peptone
  • Glycerol
  • Bacto Agar
  • Distilled water

Protocol

  • Weigh out and add ingredients with constant stirring:
    • Bacto-yeast extract (1%) 10g
    • Bacto-peptone (2%) 20g
    • Glycerol (3% [v/v]) 30mL
    • Bacto Agar (2%) 20g
    • Distilled water 970mL
  • Autoclave

References

Burke, D. Dawson, D. & Sterns, T. 2000 Methods in Yeast Genetics: A Cold Spring Harbor Laboratory Course Manual Cold Spring Harbor Laboratory Press'