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YPD liquid media


  • Bacto yeast extract
  • Bacto peptone
  • MilliQ H2O
  • Dextrose


  • Begin with approximately 700mls of MiliQ water
  • Weigh out and add ingredients with constant stirring:
    • Bacto Yeast Extract 10g
    • Bacto Peptone 20g
  • Bring up to 900mls with MiliQ water
  • Weigh out 20g of glucose/dextrose and add to 80mls of MiliQ water and bring total volume up to 100ml.
  • Autoclave separately
  • Allow to cool in 55°C water bath
  • Combine YP with glucose solution just before you are ready to use the media for the first time.


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