McClean:YPD2

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Overview

YPD agar for plates.

Reagents

  • Bacto yeast extract
  • Bacto Peptone
  • MilliQ H2O
  • Dextrose

Protocol

  • Begin with approximately 700mls of MiliQ water
  • Weigh out and add ingredients with constant stirring:
    • Bacto yeast extract 10g
    • Bacto peptone 20g
  • Weigh out and add with stirring:
    • Bacto agar 20g
  • Bring up to 900mls with MiliQ water
  • Separate YP into two 1L bottles of 450mls each
  • Weight out 20g of dextrose and add to 80mls of MiliQ water and bring volume up to 100ml total
  • Autoclave separately
  • Allow to cool in 55°C water bath
  • Combine each 450mls YP with 50mls of glucose solution

Notes

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References

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