McClean:YNB3

Overview

Yeast nitrogen base without amino acids with agar with glucose for plates. This protocol does NOT leave any room for additives.

Reagents

• Yeast Nitrogen Base (without amino acids)
• Glucose (sterile 20% solution)
• Bacto Agar
• MilliQ H₂O

Protocol

• Container 1:
  • Begin with approx. 700ml MilliQ H2O in 2L bottle
  • Add with constant stirring: 20g of Bacto Agar
  • Bring up to 800ml with MilliQ H2O
  • Autoclave
  • Allow to cool (50°F)
• Container 2:
  • Prepare 100ml solution of 20% Glucose
  • Autoclave
  • Allow to cool (50°F)

• Container 3:
  • Begin with approx. 80ml MilliQ H2O
  • Add with constant stirring 6.7g of Yeast Nitrogen base (without amino acids)
  • Bring up to 100ml with MilliQ H2O
  • Autoclave
  • Allow to cool (50°F)

Combine contents of all 3 containers and mix well

Notes

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References

Media Facility at Princeton University at the Lewis-Sigler Institute for Integrative Genomics

Contact

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