McClean:YNB3

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Overview

Yeast nitrogen base without amino acids with agar with glucose for plates. This protocol does NOT leave any room for additives.

Reagents

  • Yeast Nitrogen Base (without amino acids)
  • Glucose (sterile 20% solution)
  • Bacto Agar
  • MilliQ H2O

Protocol

  • Container 1:
    • Begin with approx. 700ml MilliQ H2O in 2L bottle
    • Add with constant stirring: 20g of Bacto Agar
    • Bring up to 800ml with MilliQ H2O
    • Autoclave
    • Allow to cool (50°F)
  • Container 2:
    • Prepare 100ml solution of 20% Glucose
    • Autoclave
    • Allow to cool (50°F)
  • Container 3:
    • Begin with approx. 80ml MilliQ H2O
    • Add with constant stirring 6.7g of Yeast Nitrogen base (without amino acids)
    • Bring up to 100ml with MilliQ H2O
    • Autoclave
    • Allow to cool (50°F)

Combine contents of all 3 containers and mix well

Notes

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  1. List troubleshooting tips here.
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References

Media Facility at Princeton University at the Lewis-Sigler Institute for Integrative Genomics

Contact

  • Who has experience with this protocol?

or instead, discuss this protocol.