McClean:LB2

Overview

Liquid LB media

Reagents

• Bacto tryptone
• Bacto yeast extract
• NaCl
• MiliQ water

Protocol

• Begin with approximately 800mls of MiliQ water
• Weigh out and add ingredients with constant stirring:
  • Bacto tryptone 10g
  • Bacto yeast extract 5g
  • NaCl 5g

• Bring up to 1L with MiliQ water
• Autoclave

Notes

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