McClean:Hygromycin B

Overview

• Protocol for making 20mL of 50mg/mL (166X) Hygromycin B stock solution
• Final concentration 300 ug/mL
• Use 6ul of stock per ml of media

Materials

1. sterile De-Ionized Water
2. Hygromycin B
3. 0.22um syringe filter
4. 30ml syringe
5. sterile 1.5ml eppie tubes

Procedure

• Fill a 50mL centrifuge tube with 20mL of sterile, DI water.
• Weigh out 1g Hygromycin B. Insert this into the tube.
• Vortex the tube vigorously until it is well-mixed.
• Filter sterilize the solution by pushing through a 0.22um syringe filter.
• Aliquot 1ml of the solution into each 1.5mL eppendorf tube. Label each tube. Store them in a labelled freezer box in the -80C freezer in 3005.

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!

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Contact

*Renee J. Szakaly: May 8, 2023

or instead, discuss this protocol.