McClean:ClonNat
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Overview
- Protocol for making 20mL of 50mg/mL (1000X) ClonNat stock solution
- Final concentration 50ug/mL
- Use 1ul stock per ml of media
Materials
- sterile De-Ionized Water
- ClonNat
- 0.22um syringe filter
- 30ml syringe
- sterile 1.5ml eppie tubes
Procedure
- Fill a 50mL centrifuge tube with 20mL of sterile, DI water.
- Weigh out 1g ClonNat. Insert this into the tube.
- Vortex the tube vigorously until it is well-mixed.
- Filter sterilize the solution by pushing through a 0.22um syringe filter.
- Aliquot 1ml of the solution into each 1.5mL eppendorf tube. Label each tube. Store them in a labelled freezer box in the -80C freezer in 3005.
Notes
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References
Contact
*Renee J. Szakaly: May 8, 2023
or instead, discuss this protocol.