- 5 ml 100x Zymo Research liquid 5-FOA
- 150ml 3.33x YNB w/o amino acids
- 50ml 20% glucose
- 50ml KS-URA (10x) amino acid solution
- 250ml 2x bacto agar
- 25mg uracil
Microwave the bacto agar to re-liquify. Allow to cool to the touch but not to solidify. Add the rest of the components (protocols can be found under media for the basic items such as YNB w/o amino acidS) to the bacto-agar. Swirl the bottom to mix the components well. If the agar is not mixing, microwave the media for short periods (30s) until everything mixes well.