Matt Gethers/CRI, Thailand/Labwork/Polyacrylamide Gels/Mayuree's Mix

From OpenWetWare
Jump to: navigation, search
Reagent Volume (Separation Layer) Volume (Stacking Layer) Notes
MilliQ 3.48 ml 3.09 ml
1.5 M Tris-Cl, pH 8.8 2.5 ml n/a Don't use this in the stacking layer; kept in cabinets above gel boxes
0.5 M Tris-Cl, pH 6.8 n/a 1.25 ml Don't use this in the separation layer;kept in cabinets above gel boxes
10% SDS 100 μl 50 μl kept in cabinets above gel boxes
40% Bis:Acrylamide 3.75 ml 0.5 ml kept in fridge in adjacent bay
TEMED 15 μl 10 μl Add this reagent last, then pour gel immediately after;kept in cabinets above gel boxes
10% APS 150 μl 100 μl Add this reagent last, then pour gel immediately after; Keep an aliquot in the -20, 0.1 g in 1 ml H20