Matt Gethers/CRI, Thailand/Labwork/Polyacrylamide Gels/Mayuree's Mix
From OpenWetWare
Jump to navigationJump to search
| Reagent | Volume (Separation Layer) | Volume (Stacking Layer) | Notes |
| MilliQ | 3.48 ml | 3.09 ml | |
| 1.5 M Tris-Cl, pH 8.8 | 2.5 ml | n/a | Don't use this in the stacking layer; kept in cabinets above gel boxes |
| 0.5 M Tris-Cl, pH 6.8 | n/a | 1.25 ml | Don't use this in the separation layer;kept in cabinets above gel boxes |
| 10% SDS | 100 μl | 50 μl | kept in cabinets above gel boxes |
| 40% Bis:Acrylamide | 3.75 ml | 0.5 ml | kept in fridge in adjacent bay |
| TEMED | 15 μl | 10 μl | Add this reagent last, then pour gel immediately after;kept in cabinets above gel boxes |
| 10% APS | 150 μl | 100 μl | Add this reagent last, then pour gel immediately after; Keep an aliquot in the -20, 0.1 g in 1 ml H20 |
