Matt Gethers/CRI, Thailand/Labwork/PCRs/PCR Taq Template
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PCR Taq Template
Rxn Mix
| Reagent | Volume/Amount |
| Paeru Genomic DNA Template | 0.5 μl |
| Taq | 0.5 μl |
| 4 μl 1 μM Stock | |
| 4 μl 1 μM Stock | |
| dNTPs | 1 μl 10 mM Stock |
| DMSO | 2.5 μl |
| Buffer | 5 μl |
| Mg2+ | 2.5 μl |
| dH20 | 30 μl |
| Total | 50 μl |
Rxn Conditions
Annealing Temperature:
Cycle (Taq)
| Step | Temperature | Duration | Notes |
| Initial Denaturation | 95oC | 2 minutes | |
| Repeat Cyclic Steps 35x | |||
| Cyclic Denaturation | 94oC | 30 Seconds | |
| Cyclic Annealing | oC | 30 Seconds | Unsure of best temp |
| Cyclic Extension | 72oC | minute | |
| Repeat Cyclic Steps 35x | |||
| Final Extension | 72oC | 10 minutes |
