Matt Gethers/CRI, Thailand/Labwork/PCRs/PCR Supermix Template

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PCR Supermix Template

Rxn Mix

Reagent	Volume/Amount
DNA Template	1.0 μl from Colony Suspension
	1.2 μl 1 μM Stock
	1.2 μl 1 μM Stock
2x PCR Master	7.5 μl
H₂0	4.1 μl
Total	15 μl

Rxn Conditions

Annealing Temperature: ^oC

Extension Time:

Cycle (Taq)

Step	Temperature	Duration	Notes
Initial Denaturation	95^oC	2 minutes
Repeat Cyclic Steps 35x
Cyclic Denaturation	94^oC	30 Seconds
Cyclic Annealing	^oC	30 Seconds
Cyclic Extension	72^oC	minute
Repeat Cyclic Steps 35x
Final Extension	72^oC	10 minutes

Run Notes

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