Matt Gethers/CRI, Thailand/Labwork/PCRs/PCR Pfu Template

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PCR PFU Template

Rxn Mix

Reagent Volume/Amount
Paeru Genomic DNA Template 0.5 μl from So Pa Pan stock
Pfu 0.5 μl
4 μl 1 μM Stock
4 μl 1 μM Stock
dNTPs 1 μl 10 mM Stock
DMSO 2.5 μl
Buffer S 5 μl
dH20 32.5 μl
Total 50 μl

Rxn Conditions

Annealing Temperature: oC

Extension Time:


Step Temperature Duration Notes
Initial Denaturation 95oC 3 minutes
Repeat Cyclic Steps 35x
Cyclic Denaturation 94oC 40 Seconds
Cyclic Annealing oC 40 Seconds
Cyclic Extension 72oC minutes
Repeat Cyclic Steps 35x
Final Extension 72oC 10 minutes

Run Notes