Matt Gethers/CRI, Thailand/Labwork/PCRs/Amplification of HmgR ORF/8.3.08 Gel Results
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8.3.08 Colony PCR Gel Results
Lane 1 | Lanes 2-5 | Lanes 6-9 |
5 μl λ ladder | 5 μl PCR product pIs001.L4 1-4 | 5 μl PCR product pIs001.L5 1-4 |
No products showed up on the gel - only primer bands. I didn't include a positive control, so I can't be sure if there was something wrong with the PCR. Given the pIs001.L5 ligation has worked before, I'm hoping there was an issue with the PCR and will repeat tomorrow.