Matt Gethers/CRI, Thailand/Labwork/PCRs/Amplification of HmgR ORF/8.3.08 Gel Results

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8.3.08 Colony PCR Gel Results

Lane 1 Lanes 2-5 Lanes 6-9
5 μl λ ladder 5 μl PCR product pIs001.L4 1-4 5 μl PCR product pIs001.L5 1-4

No products showed up on the gel - only primer bands. I didn't include a positive control, so I can't be sure if there was something wrong with the PCR. Given the pIs001.L5 ligation has worked before, I'm hoping there was an issue with the PCR and will repeat tomorrow.