Matt Gethers/CRI, Thailand/Labwork/PCRs/Amplification of HmgR ORF/8.3.08 Gel Results
8.3.08 Colony PCR Gel Results
|Lane 1||Lanes 2-5||Lanes 6-9|
|5 μl λ ladder||5 μl PCR product pIs001.L4 1-4||5 μl PCR product pIs001.L5 1-4|
No products showed up on the gel - only primer bands. I didn't include a positive control, so I can't be sure if there was something wrong with the PCR. Given the pIs001.L5 ligation has worked before, I'm hoping there was an issue with the PCR and will repeat tomorrow.