Matt Gethers/CRI, Thailand/Labwork/PCRs/Amplification of HmgR ORF/7.25.08 Master mix
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7.25.08 Master Mix
| Reagent | Volume/Amount |
| Colony Suspension | 0 μl |
| Taq | 0.81 μl |
| Primer BT2736 | 6.48 μl 1 μM Stock |
| Primer BT2737 | 6.48 μl 1 μM Stock |
| dNTPs | 1.62 μl 10 mM Stock |
| DMSO | 4.05 μl |
| Buffer | 8.1 μl |
| Mg2+ | 4.05 μl |
| dH20 | 41.31 μl |
| Total | 72.9 μl |
Aliquot 9 μl to each of 8 tubes. Add 1 μl colony suspension (1 colony in 50 μl water) to 7, then positive control (0.5 μl genomic template) to last.
