Matt Gethers/CRI, Thailand/Labwork/PCRs/Amplification of HmgR ORF/7.25.08 Master mix

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7.25.08 Master Mix

Reagent Volume/Amount
Colony Suspension 0 μl
Taq 0.81 μl
Primer BT2736 6.48 μl 1 μM Stock
Primer BT2737 6.48 μl 1 μM Stock
dNTPs 1.62 μl 10 mM Stock
DMSO 4.05 μl
Buffer 8.1 μl
Mg2+ 4.05 μl
dH20 41.31 μl
Total 72.9 μl

Aliquot 9 μl to each of 8 tubes. Add 1 μl colony suspension (1 colony in 50 μl water) to 7, then positive control (0.5 μl genomic template) to last.