Matt Gethers/CRI, Thailand/Labwork/PCRs

PCRs

Amplification of HmgA Downstream Fragment

Amplification of HmgR ORF

Amplification of HmgR ORF using HotStar

Screening for Presence and Directionality of HmgA Downstream Fragment in pUC18 (pKn001)

Screening for Presence and Directionality of HmgR Upstream Fragment in pUC18 (pKn002)

Screening for Presence and Directionality of HmgA Upstream Fragment in pUC18 (pKn003)

Screening for Presence and Directionality of HmgR Downstream Fragment in pUC18 (pKn004)

Screening for Presence of Lox/Gen Cassette in pKn004 (aka pKn006)

Templates

PCR Pfu Template

PCR Taq Template

Reagents

DNA II by Finnzyme

PCR Supermix Template