Matt Gethers/CRI, Thailand/Labwork/HmgR Isolation Protocol/8.9.08 Gel results
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8.9.08 SDS-PAGE Induction Assay
|Lane 1||Lane 2||Lane 3||Lane 4||Lane 5|
|5 μl Unstained protein marker||5 μl P7/-IPTG||5 μl P7/+IPTG||5 μl P8/-IPTG||5 μl P8/+IPTG|
Ran at 200 V for 1 hour, then stained with 0.3% Coomassie for 30 minutes. While the +IPTG lanes appear to have less protein overall, the pattern of both -/+ IPTG lanes are identical. There appears to be no induction.