Matt Gethers/CRI, Thailand/Labwork/HmgR Isolation Protocol/8.9.08 Gel results

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8.9.08 SDS-PAGE Induction Assay

Lane 1 Lane 2 Lane 3 Lane 4 Lane 5
5 μl Unstained protein marker 5 μl P7/-IPTG 5 μl P7/+IPTG 5 μl P8/-IPTG 5 μl P8/+IPTG

Ran at 200 V for 1 hour, then stained with 0.3% Coomassie for 30 minutes. While the +IPTG lanes appear to have less protein overall, the pattern of both -/+ IPTG lanes are identical. There appears to be no induction.