Matt Gethers/CRI, Thailand/Labwork/HmgR Isolation Protocol/8.1.08 OD Log

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8.1.08 OD Log

(1/20 dilution of culture)

Time pIs001.P1 pIs001.P5 pIs001.P6
0840 Placed in Shaker
1030 0.033 0.161 0.182

I diluted 50 μl of culture in 950 μl LB and pipetted up and down. Multiply each value by 20 to get actual OD. The P5 and P6 cultures are already way overgrown. I'm diluting them back 1/10 (P1 1/2) and growing up for 20-25 minutes.

1/20 Dilutions

Time pIs001.P1 pIs001.P5 pIs001.P6
1050 Dilutions Placed in Shaker
1115 0.023 0.024 0.048