Matt Gethers/CRI, Thailand/Labwork/HmgR Isolation Protocol/8.1.08 Gel Results
8.1.08 SDS-PAGE Induction Assay
|Lane 1||Lane 2||Lane 3||Lane 4||Lane 5||Lane 6||Lane 7||Lane 8||Lane 9|
|5 μl Unstained protein marker||5 μl P5/Pellet/-IPTG||5 μl P5/Pellet/+IPTG||5 μl P5/Supernatant/-IPTG||5 μl P5/Supernatant/+IPTG||5 μl P6/Supernatant/-IPTG||5 μl P6/Supernatant/+IPTG||5 μl P1/Supernatant/-IPTG||5 μl P1/Supernatant/+IPTG|
Ran for 1 hour at 200 V. Stained for 40 minutes in 0.3% Coomassie, destained for ~40 minutes in destaining solution. This is basically a repeat of what I did yesterday, except the cultures I started for P5 and P6 were inoculated with multiple colonies.