Matt Gethers/CRI, Thailand/Labwork/HmgR Isolation Protocol/7.29.08 OD Log
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7.29.08 OD Log
(1/10 dilution of culture)
Time | pIs001.P5 1 | pIs001.P5 2 | pIs001.P6 1 | pIs001.P6 2 |
0920 | Placed in Shaker | |||
1300 | 0.074 | 0.099 | 0.131 | 0.122 |
Most are overgrown, so diluted 1/3 and replaced for 25 minutes.
(1/10 Dilutions)
Time | pIs001.P5 1 | pIs001.P5 2 | pIs001.P6 1 | pIs001.P6 2 |
1345 | 0.053 | 0.099 | 0.071 | 0.076 |
Removed cultures at 1345 to induce.