Matt Gethers/CRI, Thailand/Labwork/Digests/SphI

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Reaction Mix

Reagent Volume
Template 10 μl
SphI 0.5 μl
Fast Digest Buffer 2 μl
H20 7.5 μl
Total 20 μl

Reaction Conditions

Incubate mixture at 37oC for 30 minutes and inactivate at 65oC for 15 minutes on the heat block.

Run Notes


Used SphI to digest pKn004.P1. Used 5 μl pKn004, 0.5 μl enzyme, 2 μl buffer, and 12.5 μl water. Incubated for 1 hour 15 minutes at 37 degrees and inactivated at 65 degrees for 15 minutes. Put in 4 degree over night.


Fast Digest SphI