Matt Gethers/CRI, Thailand/Labwork/Digests/NcoI

From OpenWetWare
Jump to: navigation, search

NcoI

Reaction Mix

Reagent Volume
Template 10 μl
NcoI 0.5 μl
Fast Digest Buffer 2 μl
H20 7.5 μl
Total 20 μl

Reaction Conditions

Incubate mixture at 37oC for 30 minutes and inactivate at 65oC for 15 minutes on the heat block.

Run Notes

6.13.08

Followed protocol as written to digest upstream fragment of HmgA.

PCR failed, so nothing to digest. See gel results here.

6.16.08

Followed protocol as written to digest upstream fragment of HmgA.

PCR failed, so nothing to digest. See gel results here.

6.17.08

Followed protocol as written to digest upstream fragment of HmgA. After digest and inactivation, I placed in the 4oC for ~1 hour rather than running directly on the gel because I forgot to digest pET-11a. See gel results here.

References

Fast Digest NcoI

Protocol for the Digest of PCR Product with NcoI