Marcel Quint:cycler programs

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unless stated otherwise, all programs start with initial annealing at 94°C for 3min followed by 30s at 94°C (first segment of cycle) and finish with 10 min final extension step at 72°C + hold at 4°C:

  1. Ta=50 for 45s, annealing 1 min, 35 cycles
  2. Ta=53 for 45s, annealing 1 min, 35 cycles
  3. Ta=55 for 45s, annealing 1 min, 35 cycles
  4. Ta=57 for 45s, annealing 1 min, 35 cycles