Map

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Mapping strategies

Goals:

  • to see in genR was inserted (length of DNA should be the length of pUC-ampR plasmid+ length of genR). Chose a restriction site found once in pUC-ampR plasmid to linearize it.
  • If genR was inserted, for the zinc-fingered transposases, we want to see WHERE the cassette was inserted, so we can measure the specificity of insertion.
    • We'll have to research where the zinc-fingered transposases bind and cut the DNA