Maheshri:Locations
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Descriptions of Locations
| Shelf | the shelves located above the media making station |
|---|---|
| Cupboard | the large glass cupboards facing the media making station |
| Side Shelf | the shelves underneath the windows |
| Chemical Hood 1 | underneath the chemical hood to the right of the main entrance |
| Chemical Hood 2 | underneath the chemical hood to the left of the main entrance |
| Drawer | the drawers underneath the media making station |
| Large Fridge (4C) | the large fridge between the large cupboards and secondary entrance |
| Small Fridge (4C) | the small fridge underneath the center bench |
| Large Freezer (-20C) | the -20C freezer next to Chemical Hood 1 |
| Small Freezer (-20C) | the small freezer underneath the right bench |
| Freezer (-80C) | the -80C freezer next to Chemical Hood 2 |
Chemicals
| Name | Location | Catalog # |
|---|---|---|
| 2-Mercaptoethanol | Shelf | |
| 2-Phenylethanol | ? | |
| 20X SSC Buffer | Shelf | |
| Acetic acid | Chemical Hood 2 | |
| Acetone | Chemical Hood 2 | |
| Adenine | Shelf | |
| Ammonium acetate | Shelf | |
| Ammonium bicarbonate | Shelf | |
| Ammonium sulfate | Shelf | |
| Ampicillin | Large Freezer (-20C) | |
| Bacto agar | Shelf | |
| Bacto yeast extract | Shelf | |
| BSA fraction V, RNase/DNase-free | Large Freezer (-20C) | |
| Buffer for pH meter | Shelf | |
| Butyl Alcohol | Chemical Hood 2 | |
| Calcium chloride dihydrate | Shelf | |
| CarGille Immersion Oil DF | Microscope room drawer | |
| Chloramphenicol | Shelf | |
| CYCLOHEXIMIDE | Large Freezer (-20C), Shelf | |
| DAPI / 4',6-diamidino-2-phenylindole | Large Freezer (-20C) | |
| D(+)-Galactose | Shelf | |
| D-Glycogen | ? | |
| Dextran sulfate | Shelf | |
| Dextrose | Shelf | |
| Dimethylsulfoxide | Shelf | |
| Dithiothreitol | Small Fridge (4C) | |
| Doxycycline | Small Fridge (4C) | |
| Ethidium bromide | Small Fridge (4C) | |
| Ethyl alcohol | Chemical Hood 2 | |
| Ethylenedinitrotetraacetic acid disodium salt | ? | |
| Formaldehyde 37% in water | Chemical Hood 2 | |
| Formamide | Chemical Hood 2 | |
| Gentamycin sulfate | Shelf | |
| Glycerin | Shelf | |
| Glycine | Shelf | |
| Hepes free acid | Shelf | |
| Hydrochloric acid | Chemical Hood 1 | |
| IPTG | Large Freezer (-20C) | |
| Isopropyl Alcohol | Chemical Hood 2 | |
| L-(+)-ArGinine Monohydrochloride | Shelf | |
| L-(+)-Histidine | Shelf | |
| L-Aspartic acid | Shelf | |
| L-Glutamic acid monosodium salt monohydrate | Shelf | |
| L-Isoleucine | Shelf | |
| L-Leucine | Shelf | |
| L-Lysine Monohydrate | Shelf | |
| L-Methionine | Shelf | |
| L-Phenylalanine | Shelf | |
| L-Threonine | Shelf | |
| L-Tryptophan | Shelf | |
| L-Tyrosine disodium salt, dihydrate | Shelf | |
| L-Valine | Shelf | |
| Lactic acid, 85% in water | Chemical Hood 2 | |
| Lithium acetate | Shelf | |
| Lithium chloride | Shelf | |
| Low Melting Agarose | Shelf | |
| Magnesium Acetate, tetrahydrate | Shelf | |
| Magnesium Chloride | Shelf | |
| Manganese Chloride, Tetrahydrate | Shelf | |
| methanol | Chemical Hood 2 | |
| MOPS | Shelf | |
| N,N-Dimethylformamide | Chemical Hood 2 | |
| NITRIC ACID | Chemical Hood 1 | |
| NP 40 | Shelf | |
| NPROTEIN A SEPHAROSE 4 FAST FLOW | ? | |
| Peptone | Shelf | |
| Petroleum ether | Chemical Hood 2 | |
| Phenol/Tris buffered solution | Small Fridge (4C) | |
| Phenolchloroform | Small Fridge (4C) | |
| Phosphoric acid | Chemical Hood 1 | |
| Piperazine1,4bis(2-ethanesulfonic acid) | Shelf | |
| PMSF | ? | |
| Poly-L-lysine Hydrobromide | ? | |
| Polyethylene Glycol 3350 | Shelf | |
| Potassium Acetate | Shelf | |
| Potassium Chloride | Shelf | |
| Raffinose, 5-Hydrate | Shelf | |
| Ribonucleic acid (RNA) | ? | |
| Rubidium chloride | Shelf | |
| Sodium Azide | Shelf | |
| Sodium Bicarbonate | Shelf | |
| Sodium Chloride | Shelf | |
| Sodium deoxycholate | ? | |
| Sodium Dodecyl Sulfate | Shelf | |
| Sodium Hydroxide | Shelf | |
| Sorbitol | Shelf | |
| SYBR Green I nucleic acid gel stain in DMSO | Large Freezer (-20C) | |
| Tris | Shelf | |
| Tris-HCl | Shelf | |
| Triton | Shelf | |
| Tryptone | Shelf | |
| Tryptophol | Shelf | |
| Tween 20 | Shelf | |
| Uracil | Shelf | |
| Urea | Shelf | |
| Vanadyl Ribonucleoside | ? | |
| Water | Shelf | |
| X-Gal | Large Freezer (-20C) | |
| YNB without amino acids | Shelf | |
| Bradford reagent | Small Fridge (4C) | |
| Orange G | Small Fridge (4C) | |
| Lyticase | Small Fridge (4C) | |
| L-Canavanine Sulfate | Small Fridge (4C) | |
| Phenol:Chloroform | Small Fridge (4C) | |
| FA lysis buffer | Small Fridge (4C) | |
| Sol I (Miniprep) | Small Fridge (4C) | |
| Sol III (Miniprep) | Small Fridge (4C) | |
| Proteinase K | Large Freezer (-20C) | |
| CelLytic | Large Freezer (-20C) | |
| Protease Inhibitor Cocktail | Large Freezer (-20C) | |
| Ligase | Large Freezer (-20C) | |
| Antarctic phosphatase | Large Freezer (-20C) | |
| DNAse I | Large Freezer (-20C) | |
| M-MulV reverse transcriptase | Large Freezer (-20C) | |
| DNA ladder | Large Freezer (-20C) | |
| Protein ladder | Large Freezer (-20C) | |
| T4 polynucleotide kinase | Large Freezer (-20C) | |
| RNAse A | Large Freezer (-20C) | |
| Yeast extract | Shelf |
Lab Supplies
| Name | Location |
|---|---|
| Microtube (1.5mL) | Cupboard |
| Tips (1mL) | Cupboard |
| Tips (300uL) | Cupboard |
| Tips (10uL) | Cupboard |
| Graduated cylinder | Cupboard |
| Beaker | Cupboard |
| Flask (25mL, 50mL, 2L)) | Cupboard |
| Flask (2.8L, wide for cell culture) | Cupboard |
| YPD plate | Cupboard |
| SD dropout plate | Cupboard |
| SPO plate | Cupboard |
| Test tube (Small) | Cupboard |
| Test tube (Large) | Cupboard |
| Test tube cap | Drawer |
| Weighing paper | Drawer |
| Weighing dishes | Drawer |
| Serological pipet (25mL) | Drawer |
| Serological pipet (10mL) | Drawer |
| Serological pipet (5mL) | Drawer |
| Petri dishes | Left window bench's drawer |
| Glass beads, acid-washed | Right window bench |
| MF75 Vacuum Filter Units | Cupboard |
| Falcon tube (50mL) | Cupboard |
| Falcon tube (15mL) | Cupboard |
| Syringe | Drawer |
| Syringe filter (0.45um, 0.2um) | Cupboard |
| PCR tube | Cupboard |
| Racks | Side Shelf |
| Tips (Autoclaved) | Side Shelf |
| Toothpick (Autoclaved) | Side Shelf |
| Sticks (Autoclaved) | Side Shelf |
| Deep well plate (Autoclave) | Side Shelf |
| Reservoir of multi-channel pipettes | Cupboard |
| Gloves | Cupboard |
| Amino acid solution (dropout) | Large Fridge (4C) |
| TB amp plate | Large Fridge (4C) |
| LB plate | Large Fridge (4C) |
| 5' FOA plate | Large Fridge (4C) |
| YPD G418 plate | Large Fridge (4C) |
| Bottles | Cupboard |
| 96 well plate | Right sink |
| SDS PAGE equipment | Shelf next to the whiteboard |
| Western blot equipment | Shelf next to the whiteboard |
| Tool box | Side Shelf |
| semi-micro cuvettes | Side Shelf |
| Aluminum wrap | Shelf next to the whiteboard |
| Plastic wrap | Shelf next to the whiteboard |
Stocks and Common Reagents
| Name | Location |
|---|---|
| Bacteria stock | Freezer (-80C) |
| Yeast stock | Freezer (-80C) |
| Vector stock | Large Freezer (-20C) |
| dNTP mix | Large Freezer (-20C) |
| ssDNA | Large Freezer (-20C) |
| Ampicillin stock | Large Freezer (-20C) |
| Restriction Enzyme | Large Freezer (-20C) |
| Restriction Enzyme Buffer | Large Freezer (-20C) |
| Poly lysine | Large Freezer (-20C) |
| TOP 10 competent cell | Freezer (-80C) |
| K12 competent cell | Freezer (-80C) |
