M13 labeling

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M13 labeling

In the Juenger Lab, we often use M13 universal primers in the development of length polymorphism makers.

This is the m13 sequence that we use: TGT AAA ACG ACG GCC AGT

Example of forward primer format for ordering: TGT AAA ACG ACG GCC AGT CTG CTC TGC TTG GTG ATC GT

The m13 sequence is pasted right in front of the forward primer sequence.