Luckau Protocols:TAE

TAE	Tara K. Luckau's Home Page Conservation Genetics Lab Notebook Tara's Protocols

Purpose

TAE is a commonly used buffer for making and running DNA agarose gels. It is cheap to make, easy to store, and buffers well under higher running voltages.

It is convenient to make a stock solution of 50x TAE for easy storage.

Protocol

To make 500mL of 50x TAE

(2M Tris, 2M acetic acid, 50mM EDTA):

• dissolve 121 g Tris-base (FW - 121.14) in about 300 mL MilliQ H₂O
  • Solubility in water: ~50g / 100mL (@ 25 °C)
• add 28.55 mL glacial acetic acid
• add 50 mL 0.5M EDTA (pH 8.0)
• add MilliQ H₂O to bring total volume to 500mL

Do not autoclave (I don't know why)

To make 1L of 1x TAE from 50x TAE

• 20mL 50x TAE + 980 NanoPure H₂O

Sources

• OpenWetWare's Materials TAE page
• OpenWetWare's User, Silver Lab TAE
• About.com's Biotech page