Livesey:Antibodies

Primary Antibodies

||class="wikitable" align=center border=1 cellpadding="2" ||Antibody||Species||IHC||Western||Chip||Storage||Company||Cat no.||IHC Notes || ||anti tubulin beta 3||Rabbit||1:2000||1:5000|| - ||+4||Covance||PRB-435P|| ||anti tubulin beta 3||Mouse||1:100|| - || - ||-20||Chemicon||mab1637|| ||Brdu||Rat||1:40 - 1:200|| - || - ||-20||Abcam||ab6326|| ||Brn2||Rabbit|| - ||1:500-1:1000|| - ||-20||Active Motif||160-81678598||tried at 1:500. Has worked but could try a higher concentration|| ||humanCoup tf1||Rabbit|| - ||1:100-1:250|| - ||-20||Abcam||Ab11954|| ||72.5B10 / Er81||Mouse|| - || - || - ||+4||Dev studies hybridoma||72.5B10||tried it at 1:100, doesnt work that well|| ||Ezh2||Rabbit|| - || NA || - ||-20||Abcam||Ab3748|| ||Fgfr2(iiib)||Rat|| - || - || - ||-20||R&D Systems||mab7161|| ||Gad67||Mouse||1:2000||1:2500-1:5000|| - ||+4||Chemicon||mab5406|| ||GFAP||Chicken||1:50|| - || - ||-20||Abcam||ab4674|| ||Histone H3 di methyl K9||Rabbit||1:500-1:2000||1:500||1:500||-20||Abcam||ab7312|| ||Histone H3 tri methyl K4||Rabbit||1:2000-1:5000||1:2000-1:5000||1:1000||-20||Abcam||ab8580|| ||Histone H3 tri methyl K27||Rabbit||1:200||1:500|| - ||-20||Abcam||ab6002|| ||Ki67||Mouse||1:100|| - || - ||-20||BD||K7820-050|| ||Lhx/lim2||Rabbit||1:200-1:1000|| - || - ||-20||Chemicon||ab5756|| ||Map kinase 1/2||Rabbit||1:500-1:1000|| - || - ||-20||Upstate||23058|| ||Nestin||Mouse||1:200-1:2000||1:200-1:2000||1:200-1:2000||-20||Abcam||ab6142|| ||Nestin||Mouse||1:500-1:5000|| - || - ||-20||Chemicon||mab353|| ||Nestin||Rabbit||1:200-1:1000|| - || - ||-20||Santa Cruz Biotech||sc20978|| ||Otx-5f5 / Otx1||Mouse|| - || - || - ||+4||Dev Studies hybridoma||Otx-5f5||works at 1:100|| ||Pax6||Rabbit||1:1000||1:500-1:1000|| - ||-20||Chemicon||ab5409|| ||Phospho-Akt||Mouse||1:50-1:100|| - || - ||-20||Cell signalling technology||9277|| ||Prox1||Rabbit||1:1000-1:5000||1:2500|| - ||-20||Covance||PRB-238C|| ||Prox1||Rabbit||1:5000||1:2500|| - ||-20||Chemicon||ab5475|| ||Rat 401 (Nestin)||Mouse|| - || - || - ||+4||Developmental Studies Hybridoma Bank||Rat-401|| ||Reelin||Mouse||1:100|| - || - ||+4||Chemicon||mab5364||Works really well at 1:100, an excellent antibody|| ||ROR beta||Rabbit||1:100-1:200|| - || - ||+4||Abcam||ab13113||tried at 1:200, hasnt worked that well|| ||Smad4||Rabbit|| - ||1:1000|| - ||+4||Santa Cruz Biotech||sc-7966|| ||Tuj1||Mouse||1:500|| - || - ||-20||Abcam||ab14545||Works up to 1:1000|| ||Nurr1||Rabbit||1:500|| - || - ||+4||Santa Cruz||sc-990||works well at 1:500|| ||Androgen Receptor||Rabbit|| - || 4ug/ml || - ||-20||Abcam||ab3510|| || Key

IHC = Immunohistochemistry

- = Not yet tried

Any range of dilutions are manufacturers recommendations