Lissa1:Yeast Overnight

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  1. Get liquid media (over bench). Choose the right liquid media for your yeast.
  2. Put 5 mL of liquid media in culture tubes.
  3. Be sterile!
  4. Get yeast from plate in 4 degree fridge.
  5. Swipe off 1 colony (far from other colonies) with a wooden stick.
  6. Swish stick in culture with yeast on walls of tube.
  7. Vortex briefly.
  8. Put in 30 degree room overnight on the roller with speed 7ish.