Light my XYZ: Material from Ed

• isolation of PR called 31A08 from BAC. This paper includes abs spectra from membrane preps and pH measurements of media File:Bactrhod Science00.pdf Also link for 31A08 sequence at NCBI accession number AF279106 [[1]]
• characterization of two other PRs called HOT 75m and HOT 0m that were gifts from Chon in Ed's lab on 080805 File:Proteorhodops Nature01.pdf NCBI accession number AF349981 for HOT 75m4 [[2]] NCBI accession number AF349978 for HOT 0m [[3]]
• point mutation (Q105?) to change pH gradient created in response to light File:Spectraltuning EMBO03.pdf
• example from Archea in which pH gradient created by PR is directly hooked into chemotaxis by making chimera of PR:Che. Not at all clear that this is do-able in E. coli or some other tamed bact. File:PRCheArch biochem03.pdf
• protocol for membrane preps File:PRmembranepreps FEBS97.jpg
• protocol for abs spect in presence of trans-retinal File:RetinalAbsSpect biochem99.pdf

Currently have 31A08, HOT 75m and HOT 0m from Chon in Ed's lab as well as protease deficient strain UT5600 [[4]] to express them in.

31A08 CDS complement(50866..51615)

                    /note="light-driven proton pump; has the properties of a
                    light-driven proton pump when expressed with retinal in
                    Escherichia coli"
                    /codon_start=1
                    /evidence=experimental
                    /transl_table=11
                    /product="proteorhodopsin"
                    /protein_id="AAG10475.1"
                    /db_xref="GI:9971913"
                    /translation="MKLLLILGSVIALPTFAAGGGDLDASDYTGVSFWLVTAALLAST
                    VFFFVERDRVSAKWKTSLTVSGLVTGIAFWHYMYMRGVWIETGDSPTVFRYIDWLLTV
                    PLLICEFYLILAAATNVAGSLFKKLLVGSLVMLVFGYMGEAGIMAAWPAFIIGCLAWV
                    YMIYELWAGEGKSACNTASPAVQSAYNTMMYIIIFGWAIYPVGYFTGYLMGDGGSALN
                    LNLIYNLADFVNKILFGLIIWNVAVKESSNA"

HOT Om CDS <1..>753

                    /codon_start=1
                    /transl_table=11
                    /product="proteorhodopsin"
                    /protein_id="AAK30176.1"
                    /db_xref="GI:13560723"
                    /translation="TMGKLLLILGSVIALPTFAAGGGDLDASDYTGVSFWLVTAALLA
                    STVFFFVERDRVSAKWKTSLTVSGLVTGIAFWHYMYMRGVWIETGDSPTVFRYIDWLL
                    TVPLLICEFYLILAAATNVAAGLFKKLLVGSLVMLVFGYMGEAGIMNAWGAFVIGCLA
                    WVYMIYELWAGEGKAACNTASPAVQSAYNTMMYIIIFGWAIYPVGYFTGYLMGDGGSA
                    LNLNLIYNLADFVNKILFGLIIWNVAVKESSNA"

HOT 75m4 CDS <1..>756

                    /codon_start=1
                    /transl_table=11
                    /product="proteorhodopsin"
                    /protein_id="AAK30179.1"
                    /db_xref="GI:13560729"
                    /translation="TMGKLLLILGSAIALPSFAAAGGDLDISDTVGVSFWLVTAGMLA
                    ATVFFFVERDQVSAKWKTSLTVSGLITGIAFWHYLYMRGVWIDTGDTPTVFRYIDWLL
                    TVPLQVVEFYLILAACTSVAASLFKKLLAGSLVMLGAGFAGEAGLAPVLPAFIIGMAG
                    WLYMIYELYMGEGKAAVSTASPAVNSAYNAMMMIIVVGWAIYPAGYAAGYLMGGEGVY
                    ASNLNLIYNLADFVNKILFGLIIWNVAVKESSNA"