Lidstrom:Gene Knockouts

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Knocking out genes with pCM184_KOs

Not shown: Cre recombinase site, useful for curing antibiotic via pCM157.

Advice: if possible, remove the repetitive sequence (cccccccccccc/gggggggggggg) between the up/down stream regions and the recombination site recognized by pCM157: causes Sanger sequencing to hiccup.


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Knocking out genes with pCM433KanT