Lidstrom:Formate Assay via Formate Dehdyrogenase
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- Formate concentrations can be measured using formate dehydrogenase.
- As formate is converted to CO2, NAD+ is reduced to NADH and A340 increases.
- Janet has seen excellent linearity between Vmax and mM formate down to about 0.1mM formate.
- Prepare buffer
- make extra buffer to freeze so you don't have to re-make it in the future.
- Make formate standard(s)
- Dissolve NAD+
- Dissolve FDH
- Test linearity