Lidstrom:Bug Buster

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How much BugBuster lysis solution should I add per mL of E. coli culture?

  • Janet uses 2mL per 50mL E. coli culture in TB
  • Ceci uses 10mL per 1L of E. coli culture

How can I tell if the cells are lysed?

  • The solution/slurry should become viscous from DNA exiting the cells.