Lauffenburger:Brian Joughin

From OpenWetWare

Wikiburger top.jpg

Home        People        Research        Publications        Links        Internal       

Brian A. Joughin


Research Scientist, The David H. Koch Institute for Integrative Cancer Research at MIT


Current Research

  • Examination of the interpositional dependence of kinase specificity on substrate sequence.
  • Development of a bioinformatic method for discovering substrate motif specificity of uncharacterized kinases by integration of existing data.
  • Deriving biological insight from high-throughput quantitative phosphoproteomic mass spectrometry data.


  • 2007, Ph.D., Department of Biology, MIT
    • Research Advisors: Profs. Bruce Tidor and Michael B. Yaffe
    • Doctoral thesis: "Novel Methods in Computational Analysis and Design of Protein-Protein Interactions: Applications to Phosphoregulated Interactions"
  • 2000, B.A., Department of Biophysics, Johns Hopkins University
    • Research Advisor: Prof. Eaton Lattman


  1. Amal H, Barak B, Bhat V, Gong G, Joughin BA, Wishnok JS, Feng G, Tannenbaum SR. Shank3 mutation in a mouse model of autism leads to changes in the S-nitroso-proteome and affects key proteins involved in vesicle release and synaptic function. Mol Psychiatry. 2018 Jul 9. PubMed PMID: 29988084. Abstract
  2. Jones DS, Jenney AP, Joughin BA, Sorger PK, Lauffenburger DA. Inflammatory but not mitogenic contexts prime synovial fibroblasts for compensatory signaling responses to p38 inhibition. Sci Signal. 2018 Mar 6;11(520). Abstract
  3. Archer TC, Fertig EJ, Gosline SJ, Hafner M, Hughes SK, Joughin BA, Meyer AS, Piccolo SR, Shajahan-Haq AN. Systems Approaches to Cancer Biology. Cancer Res. 2016 Nov 18. [Epub ahead of print] PubMed PMID: 27864348. Abstract
  4. Nagel ZD, Kitange GJ, Gupta SK, Joughin BA, Chaim IA, Mazzucato P, Lauffenburger DA, Sarkaria JN, Samson LD. DNA repair capacity in multiple pathways predicts chemoresistance in glioblastoma multiforme. Cancer Res. 2016 Oct 28. pii: canres.1151.2016. [Epub ahead of print] PubMed PMID: 27793847. Abstract
  5. Hughes SK, Oudin MJ, Tadros J, Neil J, Del Rosario A, Joughin BA, Ritsma L, Wyckoff J, Vasile E, Eddy R, Phillipar U, Lussiez A, Condeelis JS, van Rheenen J, White F, Lauffenburger DA, Gertler FB. PTP1B-dependent regulation of receptor tyrosine kinase signaling by the actin-binding protein Mena. Mol. Biol. Cell. Abstract
  6. Travers T, Shao H, Joughin BA, Lauffenburger DA, Wells A, Camacho CJ. Tandem phosphorylation within an intrinsically disordered region regulates ACTN4 function. Sci. Signal. 8, ra51 (2015). Abstract
  7. Meacham CE, Lawton LN, Soto-Feliciano YM, Pritchard JR, Joughin BA, Ehrenberger T, Fenouille N, Zuber J, Williams RT, Young RA, Hemann MT. A genome-scale in vivo loss-of-function screen identifies Phf6 as a lineage-specific regulator of leukemia cell growth. Genes Dev. 2015 Mar 1;29(5):483-8. Abstract
  8. Joughin BA, Liu C, Lauffenburger DA, Hogue CWV, Yaffe MB. Protein kinases display minimal interpositional dependence on substrate sequence: potential implications for the evolution of signalling networks. Phil. Trans. R. Soc. B. 2012 Sep 19. 367 2574-2583; Abstract
  9. Grosstessner-Hain K, Hegemann B, Novatchkova M, Rameseder J, Joughin BA, Hudecz O, Roitinger E, Pichler P, Kraut N, Yaffe MB, Peters JM, Mechtler K. Quantitative phospho-proteomics to investigate the Polo-like kinase 1-dependent phospho-proteome. Mol Cell Proteomics. 2011 Nov;10(11):M111.008540. Epub 2011 Aug 21. Abstract
  10. Alexander J, Lim D, Joughin BA, Hegemann B, Hutchins JRA, Ehrenberger T, Ivins F, Sessa F, Hudecz O, Nigg EA, Fry AM, Musacchio A, Stukenberg PT, Mechtler K, Peters J-M, Smerdon S-J, Yaffe MB, Spatial Exclusivity Combined with Positive and Negative Selection of Phosphorylation Motifs Is the Basis for Context-Dependent Mitotic Signaling. Sci. Signal. 4, ra42 (2011). Abstract
  11. Naegle KM, Gymrek M, Joughin BA, Wagner JP, Welsch RE, Yaffe MB, Lauffenburger DA, White FM. PTMScout: A web resource for analysis of high-throughput post-translational proteomic studies. Mol Cell Proteomics. 2010 Nov;9(11):2558-70. Epub 2010 Jul 14. Abstract PTMScout
  12. Joughin BA, Cheung E, Karuturi RK, Saez-Rodriguez J, Lauffenburger DA, Liu ET. Cellular Regulatory Networks in Systems Biomedicine: Concepts and Perspectives. Liu ET and Lauffenburger DA, eds., Academic Press. pp. 57-108 (2009). Link
  13. Joughin BA, Naegle KM, Huang PH, Yaffe MB, Lauffenburger DA, White FM. An integrated comparative phosphoproteomic and bioinformatic approach reveals a novel class of MPM-2 motifs upregulated in EGFRvIII-expressing glioblastoma cells. Mol Biosyst. 2009 Jan;5(1):59-67. Epub 2008 Oct 30. Abstract
  14. Joughin BA, Green DF, Tidor B. Action-at-a-distance interactions enhance protein binding affinity. Protein Sci. 2005 May;14(5):1363-9. Epub 2005 Mar 31. Abstract
  15. Joughin BA, Tidor B, Yaffe MB. A computational method for the analysis and prediction of protein:phosphopeptide-binding sites. Protein Sci. 2005 Jan;14(1):131-9. Epub 2004 Dec 2. Abstract
  16. Powell DW, Rane MJ, Joughin BA, Kalmukova R, Hong JH, Tidor B, Dean WL, Pierce WM, Klein JB, Yaffe MB, McLeish KR. Proteomic identification of 14-3-3zeta as a mitogen-activated protein kinase-activated protein kinase 2 substrate: role in dimer formation and ligand binding. Mol Cell Biol. 2003 Aug;23(15):5376-87. Abstract
  17. Pereira de Araujo AF, Pochapsky TC, Joughin B. Thermodynamics of interactions between amino acid side chains: experimental differentiation of aromatic-aromatic, aromatic-aliphatic, and aliphatic-aliphatic side-chain interactions in water. Biophys J. 1999 May;76(5):2319-28. Abstract