LabName:Supplementary

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Details of Spider Silk

Cadnano designs


Sequence

scaffold

  • Scaffold of 4539NT

GATCGTTACGCTAACTATGAGGGCTGTCTGTGGAATGCTACAGGCGTTGTAGTTTGTACTGGTGACGAAACTCAGTGTTACGGTACATGGGTTCCTATT GGGCTTGCTATCCCTGAAAATGAGGGTGGTGGCTCTGAGGGTGGCGGTTCTGAGGGTGGCGGTTCTGAGGGTGGCGGTACTAAACCTCCTGAGTACGGT GATACACCTATTCCGGGCTATACTTATATCAACCCTCTCGACGGCACTTATCCGCCTGGTACTGAGCAAAACCCCGCTAATCCTAATCCTTCTCTTGAG GAGTCTCAGCCTCTTAATACTTTCATGTTTCAGAATAATAGGTTCCGAAATAGGCAGGGGGCATTAACTGTTTATACGGGCACTGTTACTCAAGGCACT GACCCCGTTAAAACTTATTACCAGTACACTCCTGTATCATCAAAAGCCATGTATGACGCTTACTGGAACGGTAAATTCAGAGACTGCGCTTTCCATTCT GGCTTTAATGAGGATTTATTTGTTTGTGAATATCAAGGCCAATCGTCTGACCTGCCTCAACCTCCTGTCAATGCTGGCGGCGGCTCTGGTGGTGGTTCT GGTGGCGGCTCTGAGGGTGGTGGCTCTGAGGGTGGCGGTTCTGAGGGTGGCGGCTCTGAGGGAGGCGGTTCCGGTGGTGGCTCTGGTTCCGGTGATTTT GATTATGAAAAGATGGCAAACGCTAATAAGGGGGCTATGACCGAAAATGCCGATGAAAACGCGCTACAGTCTGACGCTAAAGGCAAACTTGATTCTGTC GCTACTGATTACGGTGCTGCTATCGATGGTTTCATTGGTGACGTTTCCGGCCTTGCTAATGGTAATGGTGCTACTGGTGATTTTGCTGGCTCTAATTCC CAAATGGCTCAAGTCGGTGACGGTGATAATTCACCTTTAATGAATAATTTCCGTCAATATTTACCTTCCCTCCCTCAATCGGTTGAATGTCGCCCTTTT GTCTTTGGCGCTGGTAAACCATATGAATTTTCTATTGATTGTGACAAAATAAACTTATTCCGTGGTGTCTTTGCGTTTCTTTTATATGTTGCCACCTTT ATGTATGTATTTTCTACGTTTGCTAACATACTGCGTAATAAGGAGTCTTAATCATGCCAGTTCTTTTGGGTATTCCGTTATTATTGCGTTTCCTCGGTT TCCTTCTGGTAACTTTGTTCGGCTATCTGCTTACTTTTCTTAAAAAGGGCTTCGGTAAGATAGCTATTGCTATTTCATTGTTTCTTGCTCTTATTATTG GGCTTAACTCAATTCTTGTGGGTTATCTCTCTGATATTAGCGCTCAATTACCCTCTGACTTTGTTCAGGGTGTTCAGTTAATTCTCCCGTCTAATGCGC TTCCCTGTTTTTATGTTATTCTCTCTGTAAAGGCTGCTATTTTCATTTTTGACGTTAAACAAAAAATCGTTTCTTATTTGGATTGGGATAAATAATATG GCTGTTTATTTTGTAACTGGCAAATTAGGCTCTGGAAAGACGCTCGTTAGCGTTGGTAAGATTCAGGATAAAATTGTAGCTGGGTGCAAAATAGCAACT AATCTTGATTTAAGGCTTCAAAACCTCCCGCAAGTCGGGAGGTTCGCTAAAACGCCTCGCGTTCTTAGAATACCGGATAAGCCTTCTATATCTGATTTG CTTGCTATTGGGCGCGGTAATGATTCCTACGATGAAAATAAAAACGGCTTGCTTGTTCTCGATGAGTGCGGTACTTGGTTTAATACCCGTTCTTGGAAT GATAAGGAAAGACAGCCGATTATTGATTGGTTTCTACATGCTCGTAAATTAGGATGGGATATTATTTTTCTTGTTCAGGACTTATCTATTGTTGATAAA CAGGCGCGTTCTGCATTAGCTGAACATGTTGTTTATTGTCGTCGTCTGGACAGAATTACTTTACCTTTTGTCGGTACTTTATATTCTCTTATTACTGGC TCGAAAATGCCTCTGCCTAAATTACATGTTGGCGTTGTTAAATATGGCGATTCTCAATTAAGCCCTACTGTTGAGCGTTGGCTTTATACTGGTAAGAAT TTGTATAACGCATATGATACTAAACAGGCTTTTTCTAGTAATTATGATTCCGGTGTTTATTCTTATTTAACGCCTTATTTATCACACGGTCGGTATTTC AAACCATTAAATTTAGGTCAGAAGATGAAATTAACTAAAATATATTTGAAAAAGTTTTCTCGCGTTCTTTGTCTTGCGATTGGATTTGCATCAGCATTT ACATATAGTTATATAACCCAACCTAAGCCGGAGGTTAAAAAGGTAGTCTCTCAGACCTATGATTTTGATAAATTCACTATTGACTCTTCTCAGCGTCTT AATCTAAGCTATCGCTATGTTTTCAAGGATTCTAAGGGAAAATTAATTAATAGCGACGATTTACAGAAGCAAGGTTATTCACTCACATATATTGATTTA TGTACTGTTTCCATTAAAAAAGGTAATTCAAATGAAATTGTTAAATGTAATTAATTTTGTTTTCTTGATGTTTGTTTCATCATCTTCTTTTGCTCAGGT AATTGAAATGAATAATTCGCCTCTGCGCGATTTTGTAACTTGGTATTCAAAGCAATCAGGCGAATCCGTTATTGTTTCTCCCGATGTAAAAGGTACTGT TACTGTATATTCATCTGACGTTAAACCTGAAAATCTACGCAATTTCTTTATTTCTGTTTTACGTGCAAATAATTTTGATATGGTAGGTTCTAACCCTTC CATTATTCAGAAGTATAATCCAAACAATCAGGATTATATTGATGAATTGCCATCATCTGATAATCAGGAATATGATGATAATTCCGCTCCTTCTGGTGG TTTCTTTGTTCCGCAAAATGATAATGTTACTCAAACTTTTAAAATTAATAACGTTCGGGCAAAGGATTTAATACGAGTTGTCGAATTGTTTGTAAAGTC TAATACTTCTAAATCCTCAAATGTATTATCTATTGACGGCTCTAATCTATTAGTTGTTAGTGCTCCTAAAGATATTTTAGATAACCTTCCTCAATTCCT TTCAACTGTTGATTTGCCAACTGACCAGATATTGATTGAGGGTTTGATATTTGAGGTTCAGCAAGGTGATGCTTTAGATTTTTCATTTGCTGCTGGCTC TCAGCGTGGCACTGTTGCAGGCGGTGTTAATACTGACCGCCTCACCTCTGTTTTATCTTCTGCTGGTGGTTCGTTCGGTATTTTTAATGGCGATGTTTT AGGGCTATCAGTTCGCGCATTAAAGACTAATAGCCATTCAAAAATATTGTCTGTGCCACGTATTCTTACGCTTTCAGGTCAGAAGGGTTCTATCTCTGT TGGCCAGAATGTCCCTTTTATTACTGGTCGTGTGACTGGTGAATCTGCCAATGTAAATAATCCATTTCAGACGATTGAGCGTCAAAATGTAGGTATTTC CATGAGCGTTTTTCCTGTTGCAATGGCTGGCGGTAATATTGTTCTGGATATTACCAGCAAGGCCGATAGTTTGAGTTCTTCTACTCAGGCAAGTGATGT TATTACTAATCAAAGAAGTATTGCTACAACGGTTAATTTGCGTGATGGACAGACTCTTTTACTCGGTGGCCTCACTGATTATAAAAACACTTCTCAGGA TTCTGGCGTACCGTTCCTGTCTAAAATCCCTTTAATCGGCCTCCTGTTTAGCTCCCGCTCTGATTCTAACGAGGAAAGCACGTTATACGTGCTCGTCAA AGCAACCATAGTACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCG CTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTT TACGGCACCTCGACCCCAAAAAACTTGATTTGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCA CGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGGCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGAAC CACCATCAAACAGGATTTTCGCCTGCTGGGGCAAACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCAGCTGTTGCC CGTCTCACTGGTGAAAAGAAAAACCACCCTGGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGT TTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTA TGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGAATTCGAGCTCGGTACCCGGG

  • Scaffold of 5081NT

AATGCTACTACTATTAGTAGAATTGATGCCACCTTTTCAGCTCGCGCCCCAAATGAAAATATAGCTAAACAGGTTATTGACCATTTGCGAAATGTATCT AATGGTCAAACTAAATCTACTCGTTCGCAGAATTGGGAATCAACTGTTATATGGAATGAAACTTCCAGACACCGTACTTTAGTTGCATATTTAAAACAT GTTGAGCTACAGCATTATATTCAGCAATTAAGCTCTAAGCCATCCGCAAAAATGACCTCTTATCAAAAGGAGCAATTAAAGGTACTCTCTAATCCTGAC CTGTTGGAGTTTGCTTCCGGTCTGGTTCGCTTTGAAGCTCGAATTAAAACGCGATATTTGAAGTCTTTCGGGCTTCCTCTTAATCTTTTTGATGCAATC CGCTTTGCTTCTGACTATAATAGTCAGGGTAAAGACCTGATTTTTGATTTATGGTCATTCTCGTTTTCTGAACTGTTTAAAGCATTTGAGGGGGATTCA ATGAATATTTATGACGATTCCGCAGTATTGGACGCTATCCAGTCTAAACATTTTACTATTACCCCCTCTGGCAAAACTTCTTTTGCAAAAGCCTCTCGC TATTTTGGTTTTTATCGTCGTCTGGTAAACGAGGGTTATGATAGTGTTGCTCTTACTATGCCTCGTAATTCCTTTTGGCGTTATGTATCTGCATTAGTT GAATGTGGTATTCCTAAATCTCAACTGATGAATCTTTCTACCTGTAATAATGTTGTTCCGTTAGTTCGTTTTATTAACGTAGATTTTTCTTCCCAACGT CCTGACTGGTATAATGAGCCAGTTCTTAAAATCGCATAAGGTAATTCACAATGATTAAAGTTGAAATTAAACCATCTCAAGCCCAATTTACTACTCGTT CTGGTGTTTCTCGTCAGGGCAAGCCTTATTCACTGAATGAGCAGCTTTGTTACGTTGATTTGGGTAATGAATATCCGGTTCTTGTCAAGATTACTCTTG ATGAAGGTCAGCCAGCCTATGCGCCTGGTCTGTACACCGTTCATCTGTCCTCTTTCAAAGTTGGTCAGTTCGGTTCCCTTATGATTGACCGTCTGCGCC TCGTTCCGGCTAAGTAACATGGAGCAGGTCGCGGATTTCGACACAATTTATCAGGCGATGATACAAATCTCCGTTGTACTTTGTTTCGCGCTTGGTATA ATCGCTGGGGGTCAAAGATGAGTGTTTTAGTGTATTCTTTTGCCTCTTTCGTTTTAGGTTGGTGCCTTCGTAGTGGCATTACGTATTTTACCCGTTTAA TGGAAACTTCCTCATGAAAAAGTCTTTAGTCCTCAAAGCCTCTGTAGCCGTTGCTACCCTCGTTCCGATGCTGTCTTTCGCTGCTGAGGGTGACGATCC CGCAAAAGCGGCCTTTAACTCCCTGCAAGCCTCAGCGACCGAATATATCGGTTATGCGTGGGCGATGGTTGTTGTCATTGTCGGCGCAACTATCGGTAT CAAGCTGTTTAAGAAATTCACCTCGAAAGCAAGCTGATAAACCGATACAATTAAAGGCTCCTTTTGGAGCCTTTTTTTTGGAGATTTTCAACGTGAAAA AATTATTATTCGCAATTCCTTTAGTTGTTCCTTTCTATTCTCACTCCGCTGAAACTGTTGAAAGTTGTTTAGCAAAATCCCATACAGAAAATTCATTTA CTAACGTCTGGAAAGACGACAAAACTTTAGATCGTTACGCTAACTATGAGGGCTGTCTGTGGAATGCTACAGGCGTTGTAGTTTGTACTGGTGACGAAA CTCAGTGTTACGGTACATGGGTTCCTATTGGGCTTGCTATCCCTGAAAATGAGGGTGGTGGCTCTGAGGGTGGCGGTTCTGAGGGTGGCGGTTCTGAGG GTGGCGGTACTAAACCTCCTGAGTACGGTGATACACCTATTCCGGGCTATACTTATATCAACCCTCTCGACGGCACTTATCCGCCTGGTACTGAGCAAA ACCCCGCTAATCCTAATCCTTCTCTTGAGGAGTCTCAGCCTCTTAATACTTTCATGTTTCAGAATAATAGGTTCCGAAATAGGCAGGGGGCATTAACTG TTTATACGGGCACTGTTACTCAAGGCACTGACCCCGTTAAAACTTATTACCAGTACACTCCTGTATCATCAAAAGCCATGTATGACGCTTACTGGAACG GTAAATTCAGAGACTGCGCTTTCCATTCTGGCTTTAATGAGGATTTATTTGTTTGTGAATATCAAGGCCAATCGTCTGACCTGCCTCAACCTCCTGTCA ATGCTGGCGGCGGCTCTGGTGGTGGTTCTGGTGGCGGCTCTGAGGGTGGTGGCTCTGAGGGTGGCGGTTCTGAGGGTGGCGGCTCTGAGGGAGGCGGTT CCGGTGGTGGCTCTGGTTCCGGTGATTTTGATTATGAAAAGATGGCAAACGCTAATAAGGGGGCTATGACCGAAAATGCCGATGAAAACGCGCTACAGT CTGACGCTAAAGGCAAACTTGATTCTGTCGCTACTGATTACGGTGCTGCTATCGATGGTTTCATTGGTGACGTTTCCGGCCTTGCTAATGGTAATGGTG CTACTGGTGATTTTGCTGGCTCTAATTCCCAAATGGCTCAAGTCGGTGACGGTGATAATTCACCTTTAATGAATAATTTCCGTCAATATTTACCTTCCC TCCCTCAATCGGTTGAATGTCGCCCTTTTGTCTTTGGCGCTGGTAAACCATATGAATTTTCTATTGATTGTGACAAAATAAACTTATTCCGTGGTGTCT TTGCGTTTCTTTTATATGTTGCCACCTTTATGTATGTATTTTCTACGTTTGCTAACATACTGCGTAATAAGGAGTCTTAATCATGCCAGTTCTTTTGGG TATTCCGTTATTATTGCGTTTCCTCGGTTTCCTTCTGGTAACTTTGTTCGGCTATCTGCTTACTTTTCTTAAAAAGGGCTTCGGTAAGATAGCTATTGC TATTTCATTGTTTCTTGCTCTTATTATTGGGCTTAACTCAATTCTTGTGGGTTATCTCTCTGATATTAGCGCTCAATTACCCTCTGACTTTGTTCAGGG TGTTCAGTTAATTCTCCCGTCTAATGCGCTTCCCTGTTTTTATGTTATTCTCTCTGTAAAGGCTGCTATTTTCATTTTTGACGTTAAACAAAAAATCGT TTCTTATTTGGATTGGGATAAATAATATGGCTGTTTATTTTGTAACTGGCAAATTAGGCTCTGGAAAGACGCTCGTTAGCGTTGGTAAGATTCAGGATA AAATTGTAGCTGGGTGCAAAATAGCAACTAATCTTGATTTAAGGCTTCAAAACCTCCCGCAAGTCGGGAGGTTCGCTAAAACGCCTCGCGTTCTTAGAA TACCGGATAAGCCTTCTATATCTGATTTGCTTGCTATTGGGCGCGGTAATGATTCCTACGATGAAAATAAAAACGGCTTGCTTGTTCTCGATGAGTGCG GTACTTGGTTTAATACCCGTTCTTGGAATGATAAGGAAAGACAGCCGATTATTGATTGGTTTCTACATGCTCGTAAATTAGGATGGGATATTATTTTTC TTGTTCAGGACTTATCTATTGTTGATAAACAGGCGCGTTCTGCATTAGCTGAACATGTTGTTTATTGTCGTCGTCTGGACAGAATTACTTTACCTTTTG TCGGTACTTTATATTCTCTTATTACTGGCTCGAAAATGCCTCTGCCTAAATTACATGTTGGCGTTGTTAAATATGGCGATTCTCAATTAAGCCCTACTG TTGAGCGTTGGCTTTATACTGGTAAGAATTTGTATAACGCATATGATACTAAACAGGCTTTTTCTAGTAATTATGATTCCGGTGTTTATTCTTATTTAA CGCCTTATTTATCACACGGTCGGTATTTCAAACCATTAAATTTAGGTCAGAAGATGAAATTAACTAAAATATATTTGAAAAAGTTTTCTCGCGTTCTTT GTCTTGCGATTGGATTTGCATCAGCATTTACATATAGTTATATAACCCAACCTAAGCCGGAGGTTAAAAAGGTAGTCTCTCAGACCTATGATTTTGATA AATTCACTATTGACTCTTCTCAGCGTCTTAATCTAAGCTATCGCTATGTTTTCAAGGATTCTAAGGGAAAATTAATTAATAGCGACGATTTACAGAAGC AAGGTTATTCACTCACATATATTGATTTATGTACTGTTTCCATTAAAAAAGGTAATTCAAATGAAATTGTTAAATGTAATTAATTTTGTTTTCTTGATG TTTGTTTCATCATCTTCTTTTGCTCAGGTAATTGAAATGAATAATTCGCCTCTGCGCGATTTTGTAACTTGGTATTCAAAGCAATCAGGCGAATCCGTT ATTGTTTCTCCCGATGTAAAAGGTACTGTTACTGTATATTCATCTGACGTTAAACCTGAAAATCTACGCAATTTCTTTATTTCTGTTTTACGTGCAAAT AATTTTGATATGGTAGGTTCTAACCCTTCCATTATTCAGAAGTATAATCCAAACAATCAGGATTATATTGATGAATTGCCATCATCTGATAATCAGGAA TATGATGATAATTCCGCTCCTTCTGGTGGTTTCTTTGTTCCGCAAAATGATAATGTTACTCAAACTTTTAAAATTAATAACGTTCGGGCAAAGGATTTA ATACGAGTTGTCGAATTGTTTGTAAAGTCTAATACTTCTAAATCCTCAAATGTATTATCTATTGACGGCTCTAATCTATTAGTTGTTAGTGCTCCTAAA GATATTTTAGATAACCTTCCTCAATTCCTTTCAACTGTTGATTTGCCAACTGACCAGATATTGATTGAGGGTTTGATATTTGAGGTTCAGCAAGGTGAT GCTTTAGATTTTTCATTTGCTGCTGGCTCTCAGCGTGGCACTGTTGCAGGCGGTGTTAATACTGACCGCCTCACCTCTGTTTTATCTTCTGCTGGTGGT TCGTTCGGTATTTTTAATGGCGATGTTTTAGGGCTATCAGTTCGCGCATTAAAGACTAATAGCCATTCAAAAATATTGTCTGTGCCACGTATTCTTACG
CTTTCAGGTCAGAAGGGTTCTATCTCTGTTGG

Staple

Staple of 4539NT
Start End Sequence
1[56] 3[63] CAGCAGGCCGAGGTGCGGGCGCTA
1[90] 3[95] AAATCGTGAACCATCGCTGCGC
1[115] 3[127] AAAAGAATAGCCCAAAACCGTAATGCGCC
2[47] 4[32] ACTAAATCGGAACCCTCAAGCGGTAAGAGTCT
2[79] 4[64] TCAAGTTTTTTGGGGTGAAAATCCGTGTTTTT
2[111] 4[96] GCGATGGCCCACTACGGCAAAATCTAGACAGG
2[143] 4[128] CAACGTCAAAGGGCGAGAGATAGGGGAGCTAA
3[24] 8[32] GCGAGAAAGGAAGGGATAGTCTTTCTTGCCTGGACTTTAC
3[64] 8[64] GGGCGCTGACGTGGCACTTGCTGGTAGATAAT
3[96] 8[96] GTAACCACATAGAACCCAGCCATTAGCACTAA
3[128] 8[128] GCTACAGGCAGTAATAATACCTACAATTGAGG
3[144] 0[144] TATGGTTGCTTAACGTGGACTCTTGTTCCAGTTCGTTGCGCTCA
4[31] 7[31] GTCCATCATAACATCAAATGCGCGGAACCACC
4[63] 7[63] ATAATCAGCTATCGGCCAGACAATGCGGTCAG
4[79] 0[65] CCTGAGAATGTTTGATGGTCGGGGAGAGGCGGTTTGC
4[95] 7[95] AACGGTACATTACCGCCTTCTGACCACGCTGA
4[111] 0[97] AGGGATTTCCTTATAAATCGTGCCAGCTGCATTAATG
4[127] 7[127] ACAGGAGGTCATGGAAAAAGGGACAAAGCATC
4[154] 6[144] CCTCGTTAGAAGCTCAATCGTCGATTCACCAGT
5[16] 8[16] ATTAGTAAAACTGATATACCGAACCGACAACT
5[48] 2[48] AACTCAAATGAGGCCAAAAGGAGCCGTAAAGC
5[80] 2[80] AGAACAATGCCAGAATAGCGGTCACACCCAAA
5[112] 2[112] AAAAACGCCCGATTAACCGCGCTTCTATCAGG
6[47] 0[33] ATGGCTATAGAAAGCGCCGAGTAACCACGCTGGGTTTTTCTTTTCAC
6[143] 0[129] CACACGACGCGCGTACTCAGAGCGGTTGAGTGCTGCCCGCTTTCCAG
7[32] 5[47] AGCAGAAGATAAAACAAAGTATTAAGTAGAAG
7[64] 5[79] TATTAACACCGCCTGCGCCGTCAATAATATCC
7[96] 5[111] GAGCCAGCAGCAAATGTCTTTAGGGCAACAGG
7[128] 5[143] ACCTTGCTGAACCTCATTGAAAGGATTTTGAC
8[31] 10[40] AAACAATTTTTAAAAGTTTGAGTAACATTATCGAAACAAT
8[63] 10[72] ACATTTGAGAACAAAGAAACCACCAGAAGGAGAATATACA
8[87] 3[79] AGATTAGAAACAGTGCCTGAAAGCGTAAGAATGCAAGTGT
8[95] 10[104] CAACTAATTCATCATATTCCTGATTATCAGATATTGCGTA
8[119] 3[111] CTAAAATAAAAAATCTATTCTGGCCAACAGAGCACACCCG
8[127] 10[136] AAGGTTATTTCATCAATATAATCCTGATTGTTAAATTATT
9[16] 11[23] TTATTAATTGCTTTGAAGGCGAAT
9[48] 6[48] ATTTTGCGGGATTTAGGAGGTGAGATTTTTGA
9[144] 7[154] TGGATTATACTCAAATCAACAGAATATCAAACC
10[39] 15[39] AACGGATTCAAGACAATTTTTAACAGACGACG
10[71] 15[71] GTAACAGTTATATTTTATTTATCATGCAGAAC
10[103] 15[103] GATTTTCAATTTAATGTAGATTAAAGTCCTGA
10[135] 15[143] TGCACGTAAATAAGGCCCCTTAGAAATTTACGAGCATGTA
11[40] 14[40] CTGAGCAAAGACTACCAGAACGCGTTAGGCAG
11[72] 14[72] AGAAAACAAATAGTGAAGTTAATTAATCGCCA
11[104] 14[104] ATTTGAATCGATAGCTGTTTGAAAAAGCCAAC
11[136] 14[136] ATAAATCATTAATTTTGTTAAATATCATATGC
12[55] 10[48] GGTCTGAGAAGAAGATCATCGGGA
12[87] 9[87] GAAGAGTCAAATTAATGTCAGATGCGGAATTA
12[119] 9[119] AAACATAGTACCTTTTATAAAGAAGATGGCAA
13[24] 11[39] TCCAATCGCGCCTGATTATTCATTTCAATTAC
13[56] 11[71] TTTTCAAAACCTTTTAGATGAAACAAACATCA
13[88] 11[103] TGACCTAAGGTTTAACTACATTTAACAATTTC
13[120] 11[135] GTGTGATAAAACAGAATTAATGGAAACAGTAC
13[144] 10[144] AGAATAAACACGTCGCTATTAAATATATGTGAGCTACCATATCA
14[39] 16[32] AGGCATTTAAGCCTTAGCTTATCC
14[71] 16[56] TATTTAACACCCAGCTGCGCCCAATAGCAAGC
14[103] 16[88] GCTCAACAACGCTAACCCGTTTTTATTTTCAT
14[135] 16[120] GTTATACAGCCAGTTAACCAAGTACCGCACTC
15[40] 19[47] ACAATAAATATAGAAGAATCAAGAAAGTCAGACAATGAAA
15[72] 17[79] GCGCCTGTTCATTACCACAATTTT
15[104] 18[104] ACAAGAAACAAGCAAGGAGCGTCTAGAGAGAA
16[31] 13[23] GGTATTCTAAGAACGCTAAAGTAAGTAATAAGGATGCAAA
16[55] 13[55] AAATCAGACAACATGTCATGTAATAGAAAACT
16[87] 13[87] CGTAGGAATTATCAACTAATTGAGTCATCTTC
16[119] 13[119] ATCGAGAAAATAATATCCAGTATATACCGACC
16[143] 19[143] GGTATTAACAAAATAATTAACGTCTAATAACG
17[24] 12[16] AGGTTTTGTCGAGCCATTCTGTCCCTCCGGCTTAGGTTGG
17[56] 12[56] TATTTTGCAACGCCAATCAGCTAAAAATCATA
17[88] 12[88] TCTTACCAGTAGGGCTAATAGATAGACGCTGA
17[120] 12[120] CCTAATTTAATTCTTACCCATCCTATCCTTGA
17[144] 14[144] ACAGCCATATTTTCCAAGAACGGAAACCAATCACCTGTTTAGTA
18[79] 21[79] TAGACGGGCCTTTTTACACGGAATCACCGACT
19[16] 21[23] AAGCCCAACCAAAGACAGGGAGGG
19[32] 17[23] GCAAGAAAGGGTAATTGAGCGCTAATATCAGACTTGCGGG
19[64] 17[55] ACCGAAGCAGAATTAACTGAACACCCTGAACATTAGTTGC
19[96] 17[87] AGCCGAACTAACATAAAAACAGGGAAGCGCATATCCTGAA
19[104] 23[119] AAAGTTACGGTGGCAAGCAAAATCACCAGTAGCTTTTGATCAGACGAT
19[128] 17[119] AAACGCAAAAAAATGAAAATAGCAGCCTTTACTTCCAGAG
20[47] 23[55] GAAAATTCGAAATTATTCATTAAAGCCCGTATTCAGAGCC
20[63] 22[48] TTTGTCACAATCAATATAGCAATATTTTCATA
20[87] 24[72] AAAGACACAGAAAAGTCGGTCATAGCCCCCTTCCAGAGCCGGGGTCAG
20[127] 22[112] AAAATACATACATAAACAGAAGGAGTCAGACT
20[143] 22[144] ATGTTAGCAAGGCCGGAAACGTCACCATAATCAGTAGC
20[154] 18[144] TATTACGCAGTGAATACCCAAACGATTTTTTGT
21[32] 20[24] TATTGACGATATGGTTTACCAGCG
21[80] 23[87] TGAGCCATGTTTTAACGCCGCCAG
22[23] 25[23] ACCACCGGCCTCAGAATTCGGAACTTAAGAGG
22[47] 25[47] ATCAAAATCACCACCCAAACAGTTGAAGGATT
22[111] 24[104] GTAGCGCGAGGCAGGTGATACAGG
22[143] 24[136] GACAGAATAAACAAATCGTTCCAG
23[24] 22[32] CCGCCACCCTCAGAGCCACCGGAA
23[56] 20[64] GCCACCAGAACCACCAATTAGCGTATCACCGTAAGTTTAT
23[88] 20[88] CATTGACAGGAGGTTGTTTTCATCTAGAGCCACATATAAAAGAAACGC
23[120] 20[128] TGGCCTTGATATTCACCAAGTTTGCCATTAGCAAACGTAG
23[144] 26[144] AAATCCTCATTTCTGAATTTACAGGTTTAGTACCAGAACCGCCA
25[24] 26[16] CTGAGACTGACAGCCCTCATAGTT
25[32] 19[31] CCTCAAGAAATGCCCCCTGCCTATAAGGTAAACCAGAGCCTAATAAGA
25[64] 19[63] TGCTCAGTTGCCTTGAGTAACAGTGGTGAATTTTGCCATCGCTATCTT
25[72] 1[55] ACCAGGCGACTGAGTTTCGTCACCGTATTGGGCGCCAGGGTGTTTGCCC
25[96] 19[95] AGGGTTGAAGTGTACTGGTAATAATTGGGAATGGCATTTTAAGCAGAT
25[104] 1[89] TATAAGTAAGCAAGCCCAATAGGAAATCGGCCAACGCGGGTTCCG
25[128] 19[127] TATCACCGTAAGCGTCATACATGGCACCATTACCTTTAGCAACCGAGG
25[136] 0[115] TACTCAGGCCCTCAGAGCCACCACTCGGGAAACCTGTC
26[47] 1[23] CCTGTAGCATTCCACACAGTGAGACGGGCAACACCTGAGAG
26[63] 25[63] AGTACAAACTACAACGAGGATTAGCGGGGTTT
26[95] 25[95] ACCCATGTACCGTAACGATAAGTGCCGTCGAG
26[127] 25[127] CCTCATTTTCAGGGATTAGCCCGGAATAGGTG


Staple of 5081NT
Start End Sequence
1[24] 4[16] TTGAATTATTCAAATATATTTTAGTTAATGGTCAGAACGC
1[56] 3[63] AAATCAATCAATCGCATAAATAAG
1[90] 3[95] GTAAATTATATAACTAGAAAAA
1[115] 3[127] TCCCTTAGAATCCACTACCTTTACAAATT
2[47] 4[32] AACGCGAGAAAACTTTCCTTTTTTACATGTTC
2[79] 4[64] AATGCTGATGCAAATCATATGTGAAAGTAATT
2[111] 4[96] CCGGCTTAGGTTGGGTCGTCGCTAAATAAGAG
2[143] 4[128] ATCATAGGTCTGAGAGTTGAAAACTGTAATTT
3[24] 8[32] TTGAAATACCGACCGTAGTTGCTATAATATCCGCAATAGC
3[64] 8[64] AATAAACATGCGGGAGAACCAATCGCCCAATA
3[96] 8[96] GCCTGTTTGAACGCGAATTCCAAGATCAGAGA
3[128] 8[128] CTTACCAGATCAGATAACTCATCGTGAACAAA
3[144] 0[144] CAACGCTCAACAATTTATCAAAAGCTTAGATTAAGTAACAGTAC
4[31] 7[31] AGCTAATGAAGAAAAATTTTGCACGCGTCTTT
4[63] 7[63] CTGTCCAGCATGTAGAGTTTTGAAAAATAAAC
4[79] 0[65] AAAAGGTAGTGAATAACCTATCGCGCAGAGGCGAATT
4[95] 7[95] AATATAAATCCTTATCGGCGTTTTAATAAGAA
4[111] 0[97] GAGCCAGTTTAATTAATTTTCGCCTGATTGCTTTGAA
4[127] 7[127] AGGCAGAGAGTACCGCTAGAAGGCATGAAAAT
4[154] 6[144] ATATTTAACAACAAGCCGTTTTCGCGCCCAATA
5[16] 8[16] TCCTGAACCCAGCTACGCTAACGACGAAGCCC
5[48] 2[48] TTTACGAGACGACGACTAAGGCGTAGACAAAG
5[80] 2[80] GCTGTCTTGTACCGACATAATTACTATATGTA
5[112] 2[112] TTAAACCAGCATTTTCATGCGTTATTTAACCT
6[47] 0[33] TCAAGATTGTGATAAAAATAAACAAATGGAAAGAGCAAAAGAAGATG
6[143] 0[129] GCAAGCAATATAAAGCCGCCAACAATAGCGATCTTTTACATCGGGAG
7[32] 5[47] CCAGAGCCTAATTTGCATGAAATACATCCTAA
7[64] 5[79] AGCCATATTATTTATCTGAGTTAAAATAATCG
7[96] 5[111] ACGATTTTTTGTTTAAGCGCTAATAACGGGTA
7[128] 5[143] AGCAGCCTTTACAGAGGAACACCCAGAACAAG
8[31] 10[40] TATCTTACAGTTACCAGAAGGAAACCGAGGAAAATTATTC
8[63] 10[72] ATAAGAGCATAACGGAATACCCAAAAGAACTGCGATTGAG
8[87] 3[79] ACAAGAATCCAATCCAAGCGAACCTCCCGACTCCGGAATC
8[95] 10[104] GATAACCCAAGACTCCTTATTACGCAGTATGTCCAGCGCC
8[119] 3[111] GTAATTGACGTCAAAATTATCCGGTATTCTAAAGTATCAT
8[127] 10[136] GTCAGAGGGTAGAAAATACATACATAAAGGTGTGTCACAA
9[16] 11[23] CCGAACAACACCGTCAGAGCCAGC
9[48] 6[48] ACGCAATAAAGAAACACAGTTACAGCCTTAAA
9[144] 7[154] GCAACATATAAGAGAATTAACTAGAATAACATA
10[39] 15[39] ATTAAAGGGACGATTGAGAGCCGCGCCCGGAA
10[71] 15[71] GGAGGGAAATCCTCATTCAGAACCGTTTAGTA
10[103] 15[103] AAAGACAACTCTGAATCACCGGAATCAGAACC
10[135] 15[143] TCAATAGAATGGCTTTTTCATAATATTTTCAGGGATAGCA
11[40] 14[40] CCATTACCCCACCCTCGCCTTGATAGGATTAG
11[72] 14[72] AATGAAACCGCCACCCTAAAGCCAAAAGTATT
11[104] 14[104] TAGCGACAAGAGCCACTTACCGTTGCCTATTT
11[136] 14[136] GACTGTAGGCCATCTTTGATGATAAACAGTGC
12[55] 10[48] CAGAGCCAATTAGCAATTGACGGA
12[87] 9[87] CTCAGAGCCATCGATACATTCAACGCATGATT
12[119] 9[119] CCGGAACCGAATCAAGATGGTTTATAGCAAAC
13[24] 11[39] GCAGGTCATGAATTATAAAATCACCAGTAGCA
13[56] 11[71] ACAAATAAGGTAAATAGGCCGGAAACGTCACC
13[88] 11[103] AGCGCAGTAAGGGCGAGCAGCACCGTAATCAG
13[120] 11[135] CGTCATACAAATTCATTTTGCCTTTAGCGTCA
13[144] 10[144] CAGGAGTGTACTATTAGCGTTTCGCGTTTTCATTAAGTTTATTT
14[39] 16[32] GATTAGCGGAATTGCGTGGGATTT
14[71] 16[56] AAGAGGCTATCTCCAACTTTCCAGACGTTAGT
14[103] 16[88] CGGAACCTTAATTGTAATAGTTAGCGTAACGA
14[135] 16[120] CCGTATAAGTGAATTTACAACGCCTGTAGCAT
15[40] 19[47] TAGGTGTATTTCTGTAAATAATAAGAGGGTAGAATGCCAC
15[72] 17[79] CCGCCACCTTTGTCGTAAAAAAGG
15[104] 18[104] GCCACCCTCAGCCCTCTCGGTTTAGCAGGGAG
16[31] 13[23] TGCTAAACAACTTTCAGGTTGATACTCAGTACAGGTTGAG
16[55] 13[55] AAATGAATTCACCGTATCAAGAGAATTCACAA
16[87] 13[87] TCTAAAGTCTCAGAACGAAACATGGAATGGAA
16[119] 13[119] TCCACAGACAGAGCCATGCCCCCTCCAGTAAG
16[143] 19[143] TACAAACTCTTAAACACGCATAACCAAAGTAC
17[24] 12[16] AACTAAAGGGGTTTTGTAAGTATACACCAGAACCACCACC
17[56] 12[56] CGTTGAAAGAGACTCCCTCAGGAGGCCACCCT
17[88] 12[88] GGAGCCTTATTATTCTCGCCACCCCCGCCTCC
17[120] 12[120] CTTTCGAGACAGTTAACCACCCTCCAAAATCA
17[144] 14[144] GCTTGATACCGTTCGTCACCAGAGCCCAATAGGGTGCCTTGAGT
18[79] 21[79] TCACCCTCAAGAGGCACCAACTTTCCCTGACG
19[16] 21[23] TTCCATTAACCTTCATGATATTCA
19[32] 17[23] AAATACGTCAACGGCTACAGAGGCTTTGAGGAAAAGGAAC
19[64] 17[55] TAAAACGAAGCAGCGAAAGACAGCATCGGAACTTTTTTCA
19[96] 17[87] CACTCATCTTAAAGGCCGCTTTTGCGGGATCGCTCCAAAA
19[104] 23[119] TTTGACCCGCAGACGGTTGGGCTTGAGATGGTCAAAAATCGTTTAGAC
19[128] 17[119] GCGCGAAACGATATATTCGGTCGCTGAGGCTTTCAGCTTG
20[47] 23[55] CAGACCAGAACAAAGCTGCTCATTAAGAGGAATAAAAACC
20[63] 22[48] ACAGATGAACGGTGTATACGAAGGATACCACA
20[87] 24[72] CGAACTGAAAAGAATAAGGTAGAAAGATTCATTTTGCAAAAAAGCGGA
20[127] 22[112] TTAGCCGGAACGAGGCCCAGCGATAATAAAAC
20[143] 22[144] ACCTGCTCTCATTGTGAATTACCTTATTACCAGTCAGG
20[154] 18[144] CGAAATCCGCGAACGGAGATTTACCATCGCCCA
21[32] 20[24] ATCAACGTGCGCATAGGCTGGCTG
21[80] 23[87] AGAAACACTCAGAAGCAGAAGTTT
22[23] 25[23] CCAAAAGGCACTATCATTTTAATTGAAGCAAA
22[47] 25[47] TTCAACTAGACGACGAGCCCGAAATTAGAGAG
22[111] 24[104] GAACTAACAGTAAAATAGGTCTTT
22[143] 24[136] ACGTTGGGCTGCGGAACAGAAAAC
23[24] 22[32] TAACCCTCGTTTACCAATGCAGAT
23[56] 20[64] AAAATAGCGAGAGGCTCAGTTGAGAAGGCTTGGAAAGAGG
23[88] 20[88] TGCCAGAGGGGGTAATGGAACAACGTAGTAAATCAATCATAAGGGAAC
23[120] 20[128] TGGATAGCGTCCAATAAAGAAAAAAACTTTAACATGTTAC
23[144] 26[144] TCGTCATAAATTTTAAACAGTTTGCAACTAAAGATATAACAGTT
25[24] 26[16] CTCCAACACATCAATTCTACTAAT
25[32] 19[31] GGTCAGGAGACTTCAAATATCGCGTTACCCAAACATAACGAACGGGTA
25[64] 19[63] TTTTGATATTGCATCAAAAAGATTCAGTGAATATTTAGGACACCAACC
25[72] 1[55] AGAGGTCATAACCTGTTTAGCTATATTCATTTCAATTACCTCAGTACAT
25[96] 19[95] AGAGCTTAACCCTGACTATTATAGCAGAACGAATTATTACCACTAAAA
25[104] 1[89] ATTGCTGATTGACCATTAGATACATACCAAGTTACAAATGCTTCT
25[128] 19[127] CAACATGTGAGAATGACCATAAATTTAATTTCTCTACGTTTATACCAA
25[136] 0[115] TTTAAATAGATTCCCAATTCTGCGAAACAATAACGGAT
26[47] 1[23] CGAGCTGAAAAGGTGGATGAAACAAACATCAAGAATTTCAT
26[63] 25[63] ATTTTCATTTGGGGCGTACCTTTAATTGCTCC
26[95] 25[95] TTTCGCAAATGGTCAATTTTTGCGGATGGCTT
26[127] 25[127] AACGAGTAGATTTAGTATATAATGCTGTAGCT


Trials and errors

Initial design


▲Initial design of DNA silk

- Prepare ssDNA backbone via RCR. In it, red parts correspond to amorphous segments of spider silk and blue parts correspond to crystalline segments of spider silk.
- To turn DNA strands(blue parts) into a plate where DNA block would be attached, DNA oligos are added. Blue parts unconnected with oligos would form base pair with DNA origami block.
- Once DNA origami blocks are added, block-backbone structure is formed.

Idea to prevent gelation and bonding strands each other

Idea 1

- Connect Plates in each strands after reducing flexibility of DNA silk by attaching ssDNA oligomers
- Then detach ssDNA oligomers using ssDNA oligomers with fully complementary sequence

Idea 2
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A segment : not attached to DNA origami plate, B segment : attached to DNA origami plate

- Oligomer has 1A'-2A'-3A' sequence in the ordered. In picture, ssDNA 1 from plasmid 1 has 1A-1B-1A-1B and ssDNA 2 from plasmid 2 has 2A-2B-2A-2B....
- By oligomers, each strand connects with one another

Idea 3
- Make it as fabric instead of thread. Electrospinning could be used for this process.

Others
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Idea about crystalline segment

Structure of brick

1. All sides of trigonal prism are made from one scaffold.(composed of 1 strands.)
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2. 3 sides made from other 3 scaffold with 1/3 scale.(composed of 3 strands.)
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Idea 1-Rectangular plate(1)
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6bp * 42 bp * 2layer = 504bp

Idea 2-Rectangular plate(2)
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12bp * 42 bp * 2layer = 1008bp

Idea 3-Trigonal prism shaped brick(Honeycomb)


Idea 4-Trigonal prism shaped brick(Square)

About 880bp, height : 80bp

Idea 5-Thick Trigonal prism shaped brick

About 2080bp, height : 80bp

Composition of BCC(Brick-Connector Complex) or Brick complex

Brick(Red) : Connector(Blue) = 3 : 1


Brick(Red) : Connector(Blue) = 3 : 3


Brick A(Red) : Brick B(Blue) = 3 : 3


Connecting location on brick & connector

Interlocking staple         Picture of linking state             Linking state by side view

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2. Error creating thumbnail: File missing
3. Error creating thumbnail: File missing

Red : Brick, Blue : Connector, Yellow : linking staple

We marked where brick and connector are connected by staple(figures above). Scaffold was used to check stability through cando progrem.

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