nf020 - chicken embryo sb - Stage 26 - 2006.10.02
Objective Inject anterior vs posterior branch of VII to see if it labels different groups of MNs.
- Left Anterior VII DiO (may be some DiO on ganglion)
- Left Posterior VII DiI
- Right Anterior VII DiO
- Right Posterior VII DiI
Not happy with injections
Clearly projecting both caudally and rostrally. Rostral branch seems to be in a somewhat more lateral position, except for what appears to be a very thin bundle of a few axons on the lateral-most edge of the wholemount on the caudal side. Caudal bundle appears in a more medial position, except for a few thin and tightly packed axons on the more lateral side. Only got afferents on injections on one of the sides
Caudal branch clearly labels more superficial/ medial column of mn on both r4 and r5, but primarily on R4. The more lateral deep colum is also well labelled.
Profiles on the floor plate - few
Injection on one side had a bit of spill into the ganglion, and on that side, DiO seems to have labelled everything VII.
Looks like the rostral afferents labelled from rostral facial form a rather defasciulated rostral branch that seems to stop short probably near the edge of r4/r3. The caudal branch clearly has 2 branches, with the lateral seeming more defasciculated, but neither of then forms a tight bundle.
The deep/lateral motoneuron column seems to be more intensely labelled in r5, although there might be a bit of labelling in r4. The more superficial/medial group is labelled in both r4 and r5, but not as much as with DiI from the rostral branch. There is in fact very little labelling.
Some slight differences in the motoneuron pattern of labelling - it looks like caudal contributing more to motoneurons whereas rostral contributes more to afferents.