Kubke Lab:Research/CND/Records/HK024

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EmbryoName HK024- chicken embryo- Stage 18 - 9.12.14


Experiment

Objective To replicate the dye injection of HK023 but in a slightly younger embryo Dyes of different colours on Trigeminal (DiO) vs 7/8th nerve complex (DiI) to see interactions between efferents of one nerve and afferents of other. Also to practice the injection on a younger chicken (slowly moving down to stage 18-17)

Procedure

  • Trigeminal nerve bilaterally DiO crystals
  • 7th/8th nerve bilaterally DiI crystals

Notes Slight contamination that I tried to remove on the R side of the 7/8th nerve complex. Other than that a good injection, put more on 7/8th nerves than the trigeminals because I got less dye in HK023 so am trying to do the same here.

Files

Results

Facial

(Green)

  • Afferents


  • Efferents




VIII

(green)

  • Afferents


  • Efferents


  • Other


Basal plate

(red)


Summary

Questions

Future