Kubke Lab:CND/MH006
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| Cranial Nerve Development | Experiment |
Embryo details
Species:
Embryo Name: MH006
Embryo stage: 28
Staging description: Eye: Midbrain ratio, mandibular arch and limb development
Cryoprotection: Yes
Experiment details
Objective:To determine what are the best parameters to cut a stage 28 embryo.
Procedure:
Cryostat Sectioning Cryostat settings (for a more detailed protocol visit Kubke_Lab:Cryomicrotomy)
| Cryostat | Histology Lab |
| Knife | |
| Day Cut | |
| Knife Angle | Close to 0 (0.3-0.5) |
| Chamber Temp | -17 C |
| Object Temp | -17C |
| Glass Slides | Gelatin subbed |
| Plane of section | {{{Plane of section}}} |
| Number of slides | |
| Observations |
(Include in your observations, eg ,were the sections serial, was any section lost, was quality assessed, etc)
Cresyl Violet staining
For more informattion see Kubke_Lab:Nissl_Stain_Protocol
| Solution | Time | Comments |
|---|---|---|
| Defatting and rehydration step | ||
| Water | ||
| 75% alcohol | ||
| 95% alcohol | ||
| 100% alcohol 1 | ||
| 100% alcohol 2 | ||
| Xylene 1 | ||
| Xylene 2 | ||
| Xylene 3 | ||
| Xylene 2 | ||
| Xylene 1 | ||
| 100% alcohol 2 | ||
| 100% alcohol 1 | ||
| 95% alcohol | ||
| 75% alcohol | ||
| Water | ||
| Staining and differentiation step | ||
| Cresyl Violet | ||
| 50% alcohol | ||
| 70% alcohol acetic acid | ||
| 95% alcohol | ||
| 100% alcohol 1 | ||
| 100% alcohol 2 | ||
| Xylene 1 | ||
| Xylene 2 | ||
| Xylene 3 | ||
| Coverslip | ||
Comments:
