Kubke Lab:CND/MH006

From OpenWetWare
Jump to: navigation, search
Cranial Nerve Development Experiment

Embryo details


Species:
Embryo Name: MH006
Embryo stage: 28
Staging description: Eye: Midbrain ratio, mandibular arch and limb development
Cryoprotection: Yes

Experiment details


Objective:To determine what are the best parameters to cut a stage 28 embryo.
Procedure:

Cryostat Sectioning Cryostat settings (for a more detailed protocol visit Kubke_Lab:Cryomicrotomy)

Cryostat Histology Lab
Knife
Day Cut
Knife Angle Close to 0 (0.3-0.5)
Chamber Temp -17 C
Object Temp -17C
Glass Slides Gelatin subbed
Plane of section {{{Plane of section}}}
Number of slides
Observations

(Include in your observations, eg ,were the sections serial, was any section lost, was quality assessed, etc)



Cresyl Violet staining
For more informattion see Kubke_Lab:Nissl_Stain_Protocol

Solution Time Comments
Defatting and rehydration step
Water
75% alcohol
95% alcohol
100% alcohol 1
100% alcohol 2
Xylene 1
Xylene 2
Xylene 3
Xylene 2
Xylene 1
100% alcohol 2
100% alcohol 1
95% alcohol
75% alcohol
Water
Staining and differentiation step
Cresyl Violet
50% alcohol
70% alcohol acetic acid
95% alcohol
100% alcohol 1
100% alcohol 2
Xylene 1
Xylene 2
Xylene 3
Coverslip


Comments:

Results

Images

Summary