Kubke Lab:/Notebook/Cranial nerve development/2011/01/17

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  • 10.00 AM: Meeting with Fabiana and Reuben
  • 11.00 AM: Nissl stained sections cut buy Fabiana.
  • 12.30 PM: Left for the day.


  • 10:00am Group Meeting
  • 10:45am Arranged access to Histology lab
  • 11:00am Began gelatin subbing of slides.
    • During the initial cleaning of the slides there was not enough ventilation for the slides to dry properly and as a result the slides were streaky and murky. I cleaned them in acid alcohol and acetone a second time and this time placed the slides on the heating tray in the fume hood room. This dried them quick enough to remove unwanted streaks in the glass.
(Note: This is most likely a reflexion of bad cleaning --MF Kubke 05:53, 20 January 2011 (EST))
    • The bottom of the beaker I was heating to dissolve the gelatin must have got too hot as some of the gelatin denatured and stuck to the bottom of the beaker. I chose to start again making a new gelatin solution. This time I had a paper towel between the heating surface and the beaker, used a lower temperature and added the gelatin slowly to prevent clumping of the gelatin powder. This allowed the gelatin to dissolve as required. --Reuben Cutfield 20:29, 16 January 2011 (EST)
(Note: Please remember you need to control the temperature of the solution with a thermometer. You then need to adjust the temperature of the heating plate to maintain that temperature constant. If you do not know the temperature of the solution it is hard to reliably reproduce the procedure. --MF Kubke 20:55, 16 January 2011 (EST))
(Note: Changed the link to the page to their new home--MF Kubke 05:55, 20 January 2011 (EST))

Plan for the week:

Monday: Sub slides for cutting with gelatin.

Tuesday: Perform serial sectioning of embryo.

Wednesday: Nissl stain the sections. and begin analyzing.

(Note: Plan changed due to the poor completion of the sectioning task. Instead I had a meeting with Fabiana to discuss the poor results.)


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