Kubke Lab:/Notebook/Cranial nerve development/2010/11/25
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Cryostat Training, Day 2
9:30am Malisha and Reuben met at Satya's lab. Reuben resumed cutting practice (of embryo RC001) and Malisha began her cutting training (of embryo MH001).Turns were taken cutting the tissue at different thicknesses varying the technique slightly each time in an attempt to learn the properties of the cryostat.
Nissl Stain practice
5 slides containing sections from RC001 were put through a Nissl stain procedure by Reuben and Malisha to assess the quality of the sectioning. Alongside the supervisor the stained slides were viewed under a microscope and the tissue was deemed to have been destroyed. A reconsideration of the sectioning technique taught by Satya was discussed. It was decided that the sections should not be re-frozen following sectioning, rather kept at room temperature. Also a thicker section was postulated to be more suitable so that there was more biological tissue present to preserve the cytoarchitecture.