KoppLab Primer Protocol
The number of #nmoles listed on the tube when it arrives is the same as the amount of water needed to dilute the primers to 1 millimolar.
This is equal to 1,000 uM
Lab stocks are kept at this concentration.
Working concentrations: Dilute 1 ul of stock primer with 99 ul of water for 10 uM concentration.
For 100 uM at 10X the number of nmoles listed
For 500 uM add 2X the number of nmoles listed