KoppLab Primer Protocol

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Diluting Primers

The number of #nmoles listed on the tube when it arrives is the same as the amount of water needed to dilute the primers to 1 millimolar.

This is equal to 1,000 uM

Lab stocks are kept at this concentration.

Working concentrations: Dilute 1 ul of stock primer with 99 ul of water for 10 uM concentration.

Special concentrations:

For 100 uM at 10X the number of nmoles listed

For 500 uM add 2X the number of nmoles listed