Koch Lab:Protocols/Kinesin/Tubulin resuspension and polymerization/PEM-G

PEM-G

80 mM PIPES

1 mM EGTA

1 mM MgCl₂

1 mM GTP

ph 6.9

Cytoskeleton sells this in 2 parts. The first is BST01 (PEM) and lyophilized GTP BST06 that you must resuspend in PEM to a concentration of 100 mM.