Koch Lab:Protocols/Kinesin/Tubulin resuspension and polymerization/BRB80

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BRB80 buffer consists of

1 mM MgCl2
1 mM EGTA
80 mM PIPES
ph 6.9

Cytoskeleton calls this buffer PEM. When you add GTP to PEM, you get GPEM to use Cytoskeleton's lingo.