Koch Lab:Protocols/ATP

KochLab -20C Freezer Labtop Cooler

May 2009

Steve Koch 14:46, 12 May 2009 (EDT): Anthony was able to get 1 M Tris-HCl, pH 7.5 and 1M MgCl2 from Kelly (thanks, Kelly!).

• (See: User:Anthony Salvagno/Notebook/Research/2009/05/12/Microtubule creation)

• Had previously added 100 ul of water to ATP. Was supposed to add 1 ml of 100 mM Tris pH 7.5, so, need to add:
  • 800 ul of cold water
  • 100 ul of 1 M Tris.

Final concentration 100 mM ATP in 100 mM Tris-HCl Ph 7.5 Stock lyophilized powder is from cytoskeleton